ABSTRACT. Impact of small variations in temperature and humidity on the reproductive activity and survival of Aedes aegypti (Diptera, Culicidae). In short space of time increase in temperature and rainfall can affect vector populations and, consequently, the diseases for them transmitted. The present study analyzed the effect of small temperature and humidity variations on the fecundity, fertility and survival of Aedes aegypti. These parameters were analyzed using individual females at temperatures ranging from 23 to 27 °C (mean 25 °C); 28 to 32 °C (mean 30 °C) and 33 to 37 °C (mean 35 ºC) associated to 60±8% and 80±6% relative humidity. Females responded to an increase in temperature by reducing egg production, oviposition time and changing oviposition patterns. At 25 ºC and 80% relative humidity, females survived two-fold more and produced 40% more eggs when compared to those kept at 35 ºC and 80% relative humidity. However, in 45% of females kept at 35 ºC and 60% relative humidity oviposition was inhibited and only 15% females laid more than 100 eggs, suggesting that the intensity of the temperature effect was influenced by humidity. Gradual reductions in egg fertility at 60% relative humidity were observed with the increase in temperature, although such effect was not found in the 80% relative humidity at 25 º C and 30 º C. These results suggest that the reduction in population densities recorded in tropical areas during seasons when temperatures reach over 35 ºC is likely to be strongly influenced by temperature and humidity, with a negative effect on several aspects of mosquito biology.KEYWORDS. Climate changes; dengue; fecundity; fertility; mosquito. RESUMO. Impacto de pequenas variações de temperatura e umidade na atividade reprodutiva e sobrevivência de Aedes aegypti (Diptera, Culicidae). Em curto espaço de tempo, um aumento na temperatura e precipitação pode afetar a população de vetores e conseqüentemente, as doenças por eles transmitidas. Nesse estudo, analisou-se o efeito de pequenas variações na temperatura e umidade, sobre fecundidade, fertilidade e sobrevivência de Aedes aegypti. Esses parâmetros foram investigados usando-se fêmeas individuais nas temperaturas: 23-27 ° C (média 25 ° C), 28-32 ° C (média 30 ° C) e 33-37 ° C (média 35 º C) associada à umidade relativa: 60 ± 8% e 80 ± 6%. As fêmeas responderam ao aumento da temperatura com redução na produção de ovos, tempo de oviposição e mudança nos padrões de postura. A 25 º C e 80%, fêmeas sobreviveram duas vezes mais e produziram 40% mais ovos, que aquelas mantidas a 35 º C e 80%. No entanto, nos grupos a 35 º C e 60% a postura foi inibida em 45% das fêmeas e apenas 15% puseram mais de 100 ovos, sugerindo que a intensidade do efeito da temperatura seja influenciado pela umidade. Reduções graduais na fertilidade a 60% de umidade relativa foram observadas com o aumento da temperatura, embora esse efeito não tenha sido registrado na umidade de 80%, nas temperaturas de 25 º C e 30 º C. Esses resultados sugerem que a redução na densidade populaciona...

BackgroundThe continued use of chemical insecticides in the context of the National Program of Dengue Control in Brazil has generated a high selective pressure on the natural populations of Aedes aegypti, leading to their resistance to these compounds in the field. Fitness costs have been described as adaptive consequences of resistance. This study evaluated the biological and reproductive performance of A. aegypti strains and a field population resistant to temephos, the main larvicide used for controlling mosquitoes.MethodsComparative tests were performed with a resistant field population from the municipality of Arcoverde, Pernambuco State, Brazil, with a high rate of temephos resistance (RR = 226.6) and three isogenetic laboratory strains from the same origin (Araripina municipality, Pernambuco): RecR (RR = 283.6); RecRNEx (RR = 250.5), a strain under a process of resistance reversion; and RecRev (RR = 2.32), a reversed susceptible strain used as an experimental control.ResultsOur study revealed that the absence of selective pressure imposed by exposure to temephos, for five consecutive generations, led to a discrete reduction of the resistance ratio and the response of the detoxifying enzymes. Most of the 19 biological parameters were impaired in the resistant strains and field population. The analysis of the fertility life table confirmed the presence of reproductive disadvantages for the resistant individuals. Similarly, the longevity, body size, and total energetic resources were also lower for the resistant females, except for the last two parameters in the field females (Arcoverde). In contrast, the sex ratio and embryonic viability suffered no interference in all strains or population evaluated, regardless of their status of resistance to temephos.ConclusionsThe reproductive potential and survival of the resistant individuals were compromised. The parameters most affected were the larval development time, fecundity, net reproduction rate, and the generational doubling time. These fitness costs in the natural population and laboratory strains investigated are likely associated with maintaining the metabolic mechanism of resistance to temephos. Our results show that despite these costs, the highly temephos resistant populations can compensate for these losses and successfully overcome the control actions that are based on the use of chemical insecticides.

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