Escherichia coli is one of the most important etiologic agents of childhood diarrhea that represents a major public health problem in developing countries and now is being recognized as emerging enteropathogens in the well developed countries. Among the Diarrhegenic Escherichia Coli (DEC) this work was focused on the Enterohemorrhagic Escherichia Coli (EHEC) that produce Shigatoxins (Stxs). The infection is mainly transmitted through food of bovine origin such as beef and dairy products that has been often associated with outbreaks which ranged from mild diarrhea to the life-threatening hemolytic uremic syndrome. The aim of this study was to evaluate the incidence of potentially virulent STEC isolates from minced meat, some selected dairy products and from children clinical cases. Out of 360 samples, a total of 115 E.coli isolates were recovered as following 35 (30.43%) isolate from meat, 21 (18.26%) from raw milk, 31 (26.96%) from cheese and 28 (24.35%) from children stool. The identification were based on Microscopical examination, Biochemical identification and Serotyping. Twenty eight isolates were identified serologically. O26:K60 serotype 6 (21.4%) was found to be the most prevalent serotype. Screening for virulence genes (Stx 1 , Stx 2 , eaeA, hlyA) was done using multiplex PCR, which revealed detection of the target genes in 10 out of 115 (8.7%) examined samples. About 7 (70%) samples possessed eaeA gene alone; while 2 (20%) samples contained Stx 2 gene. Both Stx 1 and eaeA genes were detected in 1(10%) sample only.

This research was carried to evaluate the quality of labenah (concentrated yoghurt) sold in Suez canal province by determining some chemical and microbial properties of sixty samples, collected from different places in Ismailia and Portsaid Governorate in between December 2013 and July 2014. The chemical examination revealed that the mean value of Titratable acidity percent, total solids, fat and salt content was 1.934±0.076 %, 32.21±0.56 %, 16.93±0.60 % and 2.15±.03% respectively. The results showed that 25 % and 35 % of samples did not comply with the (GSO) and (S.D.) respectively. Bacteriological examination of samples declared that the mean value of total Mesophyilic, Psychrotophic, Coliforms and Escherichia coli count was 3.18x10 5 ± 6.1x10 4 , 9.2x10 6 ± 2.4x10 5 , 1.13x10 4 ±1.63x10 3 , and 3x10 3 ± 3.7x10 2 respectively. Salmonella were not detected in all the examined samples of labenah. Mycological examination of samples revealed that, yeast and mould count were high in all samples with mean value of 4.5x10 4 ± 9x10 3 , 5.3x10 3 ±1.4x10 3 , respectively. The results revealed that the more frequent isolated yeast was the candida albican and candida tropicalis, while the most frequent isolated molud was Aspergillus and Mucor spp. Public health importances of isolated microorganisms as well as measures for improving the quality of the product were discussed.

120 samples of pickled white soft cheese (domiata cheese) and Mish were collected from local markets in Port Said governorate, Egypt. Samples were analyzed for sodium chloride level, determination of Staph. Spp at 3% and 10% Na CL, isolation of Staphylococcus aureus at 3% and 10% NaCL and Determination of Aeromonas spp. at 3% and 10% NaCL. Results obtained revealed that the mean values of the sodium chloride percentage were 3.7 ± 0.13 % in pickled domiata cheese samples and 6.1+0.14 % in mish samples. Incidence of Staph. spp in Pickled domiata cheese was 85% at 3% NaCL and 71.6% at10% NaCL. while in mish samples was 93% at3% NaCL and 83.3% at 10%NaCL. Incidence of Aeromonas spp.at 3% NaCL was nil while at 10 % NaCL was 48.3% in pickled domiata cheese and in Mish 38.3% in.

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