The accurate diagnosis of FMD is important for eradication and controlling of disease in endemic countries involving Egypt. Vaccination was the only approach to control the FMD disease in Egypt. This study was carried out for investigation and identification of vaccination by governorate and prevailing local vaccines in cattle sera in Monofeia governorate during the period from October 2018 to May 2019. 130 serum samples were tested for FMDV. 30 samples were used as control, 50 samples collected from animals received local governorate vaccine and 50 samples from animals received local prevailing vaccine. 49 out of 130 serum samples (37.7%) were positive and 81 out of 130 serum samples (62.3%) were negative for NSP (nonstructural protein). Results were (0.0), 3(3.7%) pre-vaccination and 27 (33.33%), 18(22.22%) 1 st MPV-25(30.86%), 18 (22.22%) 2 nd MPV-15 (18.52%), 13 (16.05%) 3 rd MPV and (0.0), 9 (11.11%) 4 th MPV for serotype A. For serotype (O): 9 (11.1%), 10 (12.4%) prevaccination-36 (44.44%), 28 (34.56%) 1 st MPV-33(40.74%), 27 (33.33%) 2 nd MPV-24(29.63%), 20(24.7%) 3 rd MPV-14(17.3%), 12 (14.80%) 4 th MPV. For serotype (SAT2): 4 (4.9%), 24 (4.93%) pre-vaccination-30 (37.03%), 21 (25.93%) 1 st MPV-27(33.33%), 21 (25.93%) 2 nd MPV-18 (22.22%), 14(17.3%) 3 rd MPV-3 (3.71%), 4 (4.93%) 4 th MPV. In Conclusion. FMD controlling requires vaccines that will supply disparity of infected from vaccinated animals.
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