RHOADS, A., and E. BRENNAN. 1978. The effect of ozone on chloroplast lamellae and isolated mesophyll cells of sensitive and resistant tobacco selections. Phytopathology 68:883-886. Ozone treatment of whole tobacco leaves affected inhibition of electron transport was similar in both. Likewise, chloroplast electron transport in proportion to the degree of enzymatically-isolated leaf mesophyll cells were equally visible leaf injury resulting on either the sensitive or resistant sensitive to in vitro ozone treatment. The differential ozone selection. When chloroplasts were isolated from Bel-W3 response of Bel-W3 and Bel-B tobacco was not expressed by (ozone-sensitive) and Bel-B (ozone-resistant) tobacco leaves the isolated chloroplast lamellae or leaf mesophyll cells. and exposed to identical ozone doses in vitro, however, MATERIALS AND METHODS visibly injure Bel-B plants. Half-leaf samples from Plant material.-Two selections of cigar-wrapper ozonated and nonexposed plants were harvested tobacco were utilized in all experiments; Nicotiana immediately after each fumigation for chloroplast assay. tabacum, 'Bel-W3', which is sensitive to 03 and N. Half-leaves with midribs attached were allowed to remain Swhich is resistant. The plants were on the plants to permit evaluation of visible injury after 24 tabacum 'Bel-B', wihireitn.Tepaswrehr.
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