Plant molecules are continuously investigated to prevent and treat in ammatory and ulcerative disorders associated with the gastrointestinal tract, such as gastritis, colitis, mucositis, and ulcer. However, most of the work published is devoted to investigating the therapeutic properties of secondary plant metabolites. This work investigated the gastroprotective activity of a lipid transfer protein isolated from Morinda citrifolia L., named McLTP 1 , when orally administered to mice, from the perspective of its use as a novel peptide-based drug for the prevention and treatment of ulcerative gastric lesions. Pretreatment with McLTP 1 at different doses (4, 8, or 16 mg/kg) reduced ethanol-induced gastric lesions (p < 0.05) in 40%, 84%, and 88%, respectively. In ethanol-induced gastric lesions, it was demonstrated alterations in levels of GSH (↑100%; p < 0.05) and a reduction by 45% in the levels of MDA (p < 0.05) after McLTP 1 administration (8 mg/kg). McLTP 1 showed an anti-in ammatory effect through the modulation of the cytokines IL-10 (↑33%) and TNF-α (↓54%) and was able to reduce MPO levels (↓95%) in the gastric tissue. Besides, the gastroprotective of McLTP 1 also involves the production of nitric oxide. The present ndings reveal that McLTP 1 has a gastroprotective effect dependent, at least in part, on its antiin ammatory and antioxidant effects.
Plant molecules are continuously investigated to prevent and treat inflammatory and ulcerative disorders associated with the gastrointestinal tract, such as gastritis, colitis, mucositis, and ulcer. However, most of the work published is devoted to investigating the therapeutic properties of secondary plant metabolites. This work investigated the gastroprotective activity of a lipid transfer protein isolated from Morinda citrifolia L., named McLTP1, when orally administered to mice, from the perspective of its use as a novel peptide-based drug for the prevention and treatment of ulcerative gastric lesions. Pretreatment with McLTP1 at different doses (4, 8, or 16 mg/kg) reduced ethanol-induced gastric lesions (p < 0.05) in 40%, 84%, and 88%, respectively. In ethanol-induced gastric lesions, it was demonstrated alterations in levels of GSH (↑100%; p < 0.05) and a reduction by 45% in the levels of MDA (p < 0.05) after McLTP1 administration (8 mg/kg). McLTP1 showed an anti-inflammatory effect through the modulation of the cytokines IL- 10 (↑33%) and TNF-α (↓54%) and was able to reduce MPO levels (↓95%) in the gastric tissue. Besides, the gastroprotective of McLTP1 also involves the production of nitric oxide. The present findings reveal that McLTP1 has a gastroprotective effect dependent, at least in part, on its anti-inflammatory and antioxidant effects.
This work aimed to evaluate the activity of a lipid transfer protein isolated from Morinda citrifolia L. seeds, McLTP 1 , on the development of intestinal mucositis following irinotecan administration. McLTP 1 (0.5, 2 and 8 mg/kg, i.v.) was injected into mice 1h before irinotecan administration (75 mg/kg, i.p.; 4 days), and then for additional 6 days. Seven days after the rst dose of irinotecan, diarrhea was assessed and the intestine was removed for histological evaluation, assessment of intestinal over-contractility, measurement of myeloperoxidase (MPO), glutathione (GSH), malondialdehyde (MDA), proin ammatory cytokines and chemokine (IL-1, IL-6, and KC levels -a murine homolog of human IL-8 chemokine), analysis of cyclooxygenase 2 (COX-2), nuclear factor kappa B (NF-κB) and nitric oxide synthase (iNOS) expression. At the two highest doses, McLTP 1 administration decreased mortality and diarrhea. McLTP 1 (8 mg/kg, i.v.) signi cantly prevented irinotecan-induced intestinal damage and led to a reduction in overcontractility of the intestinal muscle (p < 0.05). Moreover, McLTP 1 decreased the MPO, IL-1β, IL-6, and KC levels signi cantly by 74.7%, 42%, 92.9%, and 95.9%, respectively. Also, the expression of COX-2, NF-κB, and iNOS was reduced. Pretreatment with McLTP 1 (8mg/kg; i.v.) signi cantly reduced the MDA level and increased the duodenum homogenates' GSH level. Our study provides a potential new therapeutic for preventing irinotecan-induced mucositis, improved clinical parameters, reduced in ammation, and oxidative damage.
A identificação do perfil epidemiológico de uma Unidade de Terapia Intensiva é fundamental no auxílio de tomada de decisões e na criação de estratégias visando o aperfeiçoamento da qualidade do serviço. Objetivo: identificar o perfil dos pacientes internados na Unidade Cardiopulmonar. Metodologia: estudo caráter descritivo retrospectivo, com corte transversal e abordagem quantitativa, realizado em um hospital de referência do Ceará. Foram analisados os dados de prontuários de janeiro a julho de 2017. Resultados: os resultados evidenciaram que a população é predominantemente do sexo masculino, idosa e procedente na maior parte da emergência, vindo também do Centro de Terapia Intensiva Pós Adulto, enfermarias, Centro Cirúrgico, Unidade Semi-intensiva e da Hemodinâmica. O tempo médio de internação foi de 14 dias. Os diagnósticos de internação mais encontrados foram pneumonia, seguida de insuficiência cardíaca congestiva, pós-operatório de troca valvar, doença pulmonar obstrutiva crônica, sepse, edema agudo pulmonar e infarto agudo do miocárdio sem supra desnivelamento do segmento ST. No período da pesquisa ocorreram mais transferências do que óbitos. O índice de óbito foi de 39,80%. Conclusão: o conhecimento desses dados é fundamental para otimizar o processo de trabalho e de cuidado, possibilitando melhor planejar ações de cuidado em saúde.
Background: Hyptis crenata is a plant of great ethnopharmacological importance widely distributed in South American countries. In Northeast Brazil, teas or infusions of its aerial parts are used in folk medicine to treat several acute and chronic inflammatory diseases. In a previous work we have demonstrated that the essential oil of H. crenata (EOHc) has an antiedematogenic effect. The aim of this work was to evaluate the effect of EOHc on cytokines secretion and cellular infiltration. Methods: Peritonitis and paw edema models induced by carrageenan were used to determine leucocyte count, myeloperoxidase (MPO) activity, nitrite, and cytokines secretion. Results: EOHc (10–300 mg/kg) significantly inhibited leucocyte migration and reduced the neutrophil count (control: 1.46 × 103 ± 0.031 × 103/mL) of the total leucocytes population in extracellular exudate (control: 2.14 × 103 ± 0.149 × 103/mL) by 15.00%, 43.29%, 65.52%, and 72.83% for the doses of 10, 30, 100, and 300 mg/kg EOHc, respectively (EC50: 24.15 mg/kg). EOHc (100 mg/kg) inhibited the increase in myeloperoxidase activity and completely blocked the increase in nitrite concentration induced by carrageenan. EOHc markedly reduced the pro-inflammatory cytokines (IL-6, MCP-1, IFN-γ, TNF-α, and IL-12p70) and increased IL-10, an anti-inflammatory cytokine (compared to control group, p < 0.05). Conclusions: This study demonstrates that EOHc has a long-lasting anti-inflammatory effect mediated through interference on MPO activity, and nitrite, and cytokines secretion. This effect, coupled with low EOHc toxicity, as far as results obtained in mice could be translated to humans, suggests that EOHc has great potentiality as a therapeutic agent.
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