Purpose: To quantify aerosol and droplets generated during noncontact tonometry (NCT) and assess the spread distance of the same. Methodology: This was an experimental study on healthy human volunteers (n=8 eyes). In an experimental setup, NCT was performed on eyes (n=8) of human volunteers under normal settings, with a single and 2 drops of lubricant. High-speed shadowgraphy, frontal lighting technique, and fluorescein analysis were used to detect the possible generation of any droplets and aerosols. Mathematical computation of the spread of the droplets was then performed. Results: In a natural setting, there was no droplet or aerosol production. Minimal splatter along with droplet ejection was observed when 1 drop of lubricant was used before NCT. When 2 drops of lubricant were instilled, a significant amount of fluid ejection in the form of a sheet that broke up into multiple droplets was observed. Some of these droplets traversed back to the tonometer. Droplets ranging from 100 to 500 µm in diameter were measured. Conclusions: There was no droplet generation during NCT performed in a natural setting. However, NCT should be avoided in conditions with high-tear volume (natural or artificial) as it would lead to droplet spread and tactile contamination.

Noninvasive ocular diagnostics demonstrate a propensity for droplet generation and present a potential pathway of distribution for pathogens such as the severe acute respiratory syndrome coronavirus 2. High-speed images of the eye subjected to air puff tonometry (glaucoma detection) reveal three-dimensional, spatiotemporal interaction between the puff and tear film. The interaction finally leads to the rupture and breakup of the tear film culminating into sub-millimeter sized droplet projectiles traveling at speeds of 0.2 m/s. The calculated droplet spread radius ( 0.5 m) confirms the likelihood of the procedure to generate droplets that may disperse in air as well as splash on instruments, raising the potential of infection. We provide a detailed physical exposition of the entire procedure using high fidelity experiments and theoretical modeling. We conclude that air puff induced corneal deformation and subsequent capillary waves lead to flow instabilities (Rayleigh–Taylor, Rayleigh–Plateau) that lead to tear film ejection, expansion, stretching, and subsequent droplet formation.

Inspired by the self-cleaning ability of lotus leaves and stickiness (towards water) of rose petals, we investigate the droplet impact dynamics on such bioinspired substrates. Impact studies are carried out with water droplets for a range of impact velocities on glass, PDMS and soft lithographically fabricated replicas of the lotus leaf and rose petals, which exhibit near identical wetting properties as that of the original biological entities. In this work, we investigate the spreading, dewetting and droplet break-up mechanisms subsequent to impact. Surprisingly, the rose petal and lotus leaf replicas manifest similar impact dynamics. The observation is extremely intriguing and counterintuitive, as rose petal and its replicas are sticky in contrast to lotus leaves. However, these observations are based on experiments performed with sessile water droplets. By contrast, in the current study, we find that rose petal replicas exhibit non-sticky behaviour at the short time scale ∼ ( O ( 10 − 3 ) )  s similar to that exhibited by lotus leaf replicas. Air entrapment in the micrometre features of bioinspired surfaces prevent frictional dissipation of droplet kinetic energy, leading to contact edge recession. We have also unveiled interesting universal physics that govern the spreading, recession of the contact edge and subsequent break-up modes (ligament or bulb-ligament) of the droplet.

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High-speed shadowgraphy showed droplets generated during flap cut with microkeratome. However, aerosolization of these droplets was unlikely because these droplets would settle on surface before aerosolization.