The content and composition of fatty acids, sterols, tocopherols, and c-oryzanol in wild rice (Zizania palustris) grown in North America were compared with those in regular brown rice (Oryza sativa L.). The lipid content of wild rice ranged from 0.7 to 1.1%, compared with 2.7% in regular brown rice. The lipids of wild rice comprised mainly linoleic (35-37%) and linolenic (20-31%) acids. Other fatty acids included palmitic (14.1-18.4%), stearic (1.1-1.3%), and oleic (12.8-16.2%). Wild rice lipids contained very large amounts of sterols, ranging from 70 g/kg for a Saskatchewan sample to 145 g/kg for Minnesota Naturally Grown Lake and River Rice. The main sterols found in an unsaponified fraction were: campesterol (14-52%), b-sitosterol (19-33%), D 5 -avenasterol (5-12%), and cycloartenol (5-12%). Some of sterols, c-oryzanols, were present as the phenolic acid esters; the amount ranged from 459 to 730 mg/kg in wild rice lipids. The largest amounts of tocopherols and tocotrienols, 3682 and 9378 mg/kg, were observed in North Western Ontario wild rice samples, whereas the lowest were 251 mg/kg in an Athabasca Alberta sample and 224 mg/kg in regular long-grain brown rice. The a isomer was the most abundant among tocopherols and tocotrienols. The results of this study showed that wild rice lipids contain large amounts of nutraceuticals with proven positive health effects.
Cereal coffee is a coffee substitute made mainly from roasted cereals such as barley and rye (60-70%), chicory (15-20%), and sugar beets (6-10%). It is perceived by consumers as a healthy, caffeine free, non-irritating beverage suitable for those who cannot drink regular coffee made from coffee beans. In presented studies, typical Polish cereal coffee brew has been subjected to the key odorants analysis with the application of gas chromatography-olfactometry (GC-O) and aroma extract dilution analysis (AEDA). In the analyzed cereal coffee extract, 30 aroma-active volatiles have been identified with FD factors ranging from 16 to 4096. This approach was also used for characterization of key odorants in ingredients used for the cereal coffee production. Comparing the main odors detected in GC-O analysis of roasted cereals brew to the odor notes of cereal coffee brew, it was evident that the aroma of cereal coffee brew is mainly influenced by roasted barley. Flavor compound identification and quantitation has been performed with application of comprehensive multidimentional gas chromatography and time-of-flight mass spectrometry (GCxGC-ToFMS). The results of the quantitative measurements followed by calculation of the odor activity values (OAV) revealed 17 aroma active compounds of the cereal coffee brew with OAV ranging from 12.5 and 2000. The most potent odorant was 2-furfurylthiol followed by the 3-mercapto-3-methylbutyl formate, 3-isobutyl-2-methoxypyrazine and 2-ethyl-3,5-dimethylpyrazine, 2-thenylthiol, 2,3-butanedione, 2-methoxy phenol and 2-methoxy-4-vinyl phenol, 3(sec-butyl)-2-methoxypyrazine, 2-acetyl-1-pyrroline, 3-(methylthio)-propanal, 2,3-pentanedione, 4-hydroxy-2,5-dimethyl-3-(2H)-furanone, (E,E)-2,4-decadienal, (Z)-4-heptenal, phenylacetaldehyde, and 1-octen-3-one.
γ-Oryzanol, a natural mixture of ferulic acid esters of triterpene alcohols and sterols, are an important bioactive components present in rice bran oil. In light of the recent increase in the popularity of wild rice among consumers, and the possibility of a direct relationship between γ-oryzanol composition and its bioactivity, the oryzanol profile of major wild rice (Zizania palustris) grown in North America was studied and compared to regular brown rice (Oryza sativa L.). A total of twenty-three γ-oryzanol components were separated, identified and quantified by HPLC coupled to an Orbitrap MS. The distribution of individual γ-oryzanols was similar for all the wild rice but significantly different from those of the regular brown rice. Unlike in the regular brown rice, a significant amount of steryl caffeate and cinnamate were found in the wild rice samples. Generally, the amounts of γ-oryzanol in the wild rice were higher compared to the regular brown rice, 1,352 vs. 688 μg/g. The results from this study showed that wild rice had a more diverse γ-oryzanol composition and the higher amounts compared to the regular brown rice.
Fatness traits, including fatty acid (FA) composition, influence the dietary value of meat products. In this study we compared the fatty acid profiles in the carcasses of two main Polish breeds, Polish Large White (PLW) and Polish Landrace (PL), and two common commercial ones, Duroc and Pietrain. The Longissimus dorsi muscle, subcutaneous and visceral fats were sampled from 85 gilts that were fed the same diets and slaughtered at the same body weight (100 kg). Several significant between-breed differences were observed in FA composition, especially in subcutaneous and abdominal fat. In all tissues, the lowest percentage of saturated fatty acids (SFA) was observed in Pietrain pigs, with the differences depending mainly on C16:0 and C18:0. In abdominal fat, the highest content of monounsaturated fatty acids was found in that of PLW, chiefly due to an elevated content of C18:1 n-9. In both adipose tissues the highest content of polyunsaturated fatty acids (PUFA) was found in Pietrain and the lowest in PLW. These differences were mainly because of the high content of C18:2 in Pietrain pigs. The existence of between-breed and tissue differences was confirmed by linear discriminant analysis, which revealed that the studied tissues can be distinguished according to FA profile with an overall prediction ability of 89.8%. It was also shown that the percentage of PUFA in the studied tissues was negatively correlated with abdominal fat weight. We conclude that the FA profile in the carcass of Pietrain pigs is the most favourable, while two Polish breeds and the Duroc breed have similar FA profiles.
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