The effects of low impact development (LID) techniques, such as green roofs and porous pavements, on the runoff and pollutant load from an apartment complex were simulated using the Site Evaluation Tool (SET). The study site was the Olympic Village, a preexisting apartment complex in Seoul, South Korea, which has a high percentage of impervious surfaces (approximately 72% of the total area). Using the SET, the effects of replacing parking lots, sidewalks and driveways (37.5% of the total area) having porous pavements and rooftops (14.5% of the total area) with green roofs were simulated. The simulation results indicated that LID techniques reduced the surface runoff, and peak flow and pollutant load, and increased the evapotranspiration and soil infiltration of precipitation. Per unit area, the green roofs were better than the porous pavements at reducing the surface runoff and pollutant loads, while the porous pavements were better than green roofs at enhancing the infiltration to soil. This study showed that LID methods can be useful for urban stormwater management and that the SET is a useful tool for evaluating the effects of LID on urban hydrology and pollutant loads from various land covers.
The 3D cell migration assay was developed for the evaluation of drugs that inhibit cell migration using high throughput methods. Wound-healing assays have commonly been used for cell migration assays. However, these assays have limitations in mimicking the in vivo microenvironment of the tumor and measuring cell viability for evaluation of cell migration inhibition without cell toxicity. As an attempt to manage these limitations, cells were encapsulated with Matrigel on the surface of the pillar, and an analysis of the morphology of cells attached to the pillar through Matrigel was performed for the measurement of cell migration. The micropillar/microwell chips contained 532 pillars and wells, which measure the migration and viability of cells by analyzing the roundness and size of the cells, respectively. Cells seeded in Matrigel have a spherical form. Over time, cells migrate through the Matrigel and attach to the surface of the pillar. Cells that have migrated and adhered have a diffused shape that is different from the initial spherical shape. Based on our analysis of the roundness of the cells, we were able to distinguish between the diffuse and spherical shapes. Cells in Matrigel on the pillar that were treated with migration-inhibiting drugs did not move to the surface of the pillar and remained in spherical forms. During the conduct of experiments, 70 drugs were tested in single chips and migration-inhibiting drugs without cell toxicity were identified. Conventional migration assays were performed using transwell for verification of the four main migration-inhibiting drugs found on the chip.
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