Değİrmencİ Karataş D., Aydin F., Aydin I., Karataş H. (2015): Elemental composition of red wines in Southeast Turkey. Czech J. Food Sci., 33: 228-236.Mineral profiles of wines and soil extracts were analysed in the five most popular international wines (Cabernet Sauvignon, Syrah, Merlot, Cot, and Tannat) and national Boğazkere, which is one of the highest-quality grapevine varieties grown in Turkey. We carried out study to establish the mineral and metal concentrations in wines from Southeast Anatolia of Turkey. The ICP-OES method was used for accurate determination of concentrations of 12 elements including Ca, Cd, Co, Cu, Fe, K, Mg, Mn, Na, Ni, Pb, and Zn. The high levels of iron, potassium, sodium, and calcium were observed in the wine samples analysed. Major elements were also abundantly found in the soil samples. Minor element contents of wine samples were compatible with results other researches. The amount of metal content in wine is an important issue. A remarkable finding of the study was that heavy metals like Pb (found below acceptable limits only in three samples), Co, and Cd were not detected. The results obtained did not exceed the maximum acceptable limits established by the Office International de la Vigne et du Vin. Our results showed that the mineral compositions of wine and soils were interrelated. The analysis of the soil samples taken from the vineyard locations showed similar results for highest composition of major minerals. The characteristics of the wines produced in the Southeast Region of Turkey were analysed for the first time.

Wild grapevine genetic diversity in southeast Turkey has not been documented to date. In the present work, in order to clarify the relationships between wild and cultivated grape accessions from southeastern Turkey, 22 nuclear and three chloroplast microsatellite loci were used on 21 wild grapevine Vitis vinifera L. ssp. sylvestris (Gmelin) and 13 cultivated grapevine Vitis vinifera ssp. sativa accessions. The number of alleles per SSR locus ranged from 4 (VVIn16) to 20 (VVIv67) and the mean allele number per locus was 10.09. Expected locus heterozygosity ranged from 0.586 (locus VVIb01) to 0.898 (locus (VVIv67)). The three cpSSR molecular markers presented variation in size both in cultivars and in wild Turkish accessions. Two size variants were detected for cpSSR3 (106 and 107 bp) for cpSSR5 (104 and 105 bp), and for cpSSR10 (115 and 116 bp). The six alleles in wild grapevines fell into three haplotypes B, C and D. A genetic structure according to accessions taxonomic status (wild or cultivated) was revealed by UPGMA analysis. This highlighted a clear separation between domesticated and wild accessions in Turkish germplasm. The results pointed out the need to further collect and characterize this wild and cultivated grapevine germplasm.

ABSTRACT. The effects of induced mutation produced by five different doses of gamma irradiation (20, 25, 30, 40, and 45 Gy) were determined using molecular approaches in Vitis vinifera cultivars, namely Thompson Seedless (Sultani Çekirdeksiz) (progenitor of seedless vinifera variety) and Kalecik Karası (one of the best quality wine grape variety of Turkey). Mutant candidates were selected through morphological observations of mutation-induced phenotypic changes during the first, second and third vegetation periods after radiation applications. Amplification studies started with 50 primers (expressed sequence tags) applied to the mutated individuals. Only 15 of these primers revealed polymorphic profiles. Twenty-two candidate mutants of Thompson Seedless and Kalecik Karası, selected based on morphological observations, were analyzed with 15 single-strand conformation polymorphism (SSCP) markers, together with 46 control plants. Polymorphic bands were rarely obtained in the SSCP analysis, and they were not reproducible.

In this study, some phytochemical properties of six seeded raisin species that are mainly cultivated in Southeastern Anatolia were investigated. Additionally, some physical and quality characteristics, phenolic contents (by LC‐MS/MS; Liquid Chromatography Tandem Mass/Mass Spectrometer System), anticholinesterase, and antioxidant capacities (DPPH; 2,2‐diphenyl‐1‐picrylhydrazyl free‐radical scavenging, ABTS; 2,2‐azinobis(3‐ethylbenzothiazoline‐6‐sulphonic acid cation‐radical scavenging activity and CUPRAC; cupric reducing antioxidant capacity) of the cultivars were investigated on ground raisins. In all three methods, the antioxidant activity values of seed extracts were determined to be higher than those of leaf and pulp extracts. Remarkably, the seed extract of Banazı Siyahı showed the highest antioxidant activity in ABTS (IC50: 4.35±0.02 μg/mL), DPPH (IC50: 10.78±0.78 μg/mL), and CUPRAC (A0.5: 9.33±0.45 μg/mL) methods. Additionally, the ethanol extracts of all pulp samples showed higher anticholinesterase activity against acetyl‐(AChE) and butyrylcholinesterase (BChE) enzymes than galantamine. According to the LC‐MS/MS results, catechin (21.362 mg analyte/g extract) and epicatechin (44.667 mg analyte/g extract) found to be quite rich in Kerküş seed extract and isoquercitrin (116.873 mg analyte/g extract) and astragalin (31.915 mg analyte/g extract) detected to be quite rich in Banazı Siyahı leaf extract. Considering the mineral content of the varieties and the soil samples they grow in, all of the grape varieties analyzed in the study was found to be rich. Based on these findings, it might be suggested that Banazı Siyahı and Kerküş varieties have potential to be utilized in pharmaceutical and food industries, due to their contents of catechins, isoquercitrin and astragalin.

This study was aimed to identify the grape cultivars growing in Diyarbakır. A total of 45 genotypes were analyzed using 7 microsatellite loci. The numbers of alleles per locus ranged between 7 and 10, whereby VVS2 and VrZAG47 had the highest and VVMD7, VrZAG62, and VrZAG79 had the lowest number of alleles. The expected and observed heterozygosity were 0.77 and 0.73, respectively. Clustering analysis was performed using the UPGMA method (Unweighted Pair-Group Method using Arithmetic means). A dendrogram was constructed based on the genetic similarity among the genotypes, which indicated 5 distinct groups, with each group involving numerous subgroups. Two additional genotypes that are used as reference genotypes around the world, Cabernet Sauvignon and Merlot, were also analyzed and were classified into a separate subgroup and differentiated from the other 43 genotypes. In conclusion, synonyms and homonyms were detected among some of the genotypes analyzed in the study.