DNAku test is one of the first direct to customer genetics testing in Indonesia. DNAku test came with a different approach as it gave their customer reports of their genetic predisposition to corresponding traits. Four months after launch, we create consumer report of this genetics testing based on DNAku questionnaire results. We applied three statistical methods, including descriptive statistics, multivariate normality test, and t-test to the demographic data of the DNAku customers. The result shows population clusters which may represent population groups that are interested in genetics testing. The questionnaire also has questions about the customer’s lifestyle. The results of these questions were processed to find the lifestyle of DNAku customers.
Hepatitis C virus (HCV) infection can cause chronic liver disease that develops into cirrhosis and liver cancer. It is estimated that are more than 170 million of th world’s population suffering from HCV. Accurate diagnosis is needed to provide appropriate early treatmen, including preventing further transmission of the virus. The purpose of this study was to construct plasmid expression of recombinant antigen for detection of anti-HCV antibodies. The antigen coding gene is designed so that is composed to epitopes that are immunodominant, sustainable and and represent HCV subtypes circulating in Indonesia and globally. Furthermore, the gene was made by synthetic DNA techniques by DNA synthesis service providers and accepted by the researchers in the form of blinding on the PUC57 plasmid to pQE80L plasmid with BamHI and HindIII cloning sites. Subcloned recombinant plasmids were then propagated on Top10 Escherichia coli cells and verified by PCR colony tecnique, restriction, and sequencing analysis. HCV recombinant antigen coding gene is 1200 bp. Cloning of these gene on the PUC57 vector produced a plasmid pUC57-HCV_ME (3910 bp) and subcloned in the pQE80L vector producing pQE80L-HCV_ME plasmid (5909bp). Based on verification results of pQE80L-HCV_ME plasmid the expression of recombinant antigen for detection of anti-HCV antibodies has been successfully constructed. AbstrakInfeksi hepatitis C virus (HCV) dapat menyebabkan penyakit hati kronis yang berkembang menjadi sirosis dan kanker hati. Diperkirakan terdapat lebih dari 170 juta penduduk dunia menderita HCV. Diagnosis yang akurat diperlukan untuk memberikan penanganan tepat secara dini, termasuk mencegah penularan virus tersebut lebih lanjut. Tujuan penelitian ini adalah mengonstruksi plasmid pengekspresi antigen rekombinan untuk deteksi antibodi anti-HCV. Gen pengode antigen tersebut dirancang sedemikian rupa sehingga tersusun atas epitop yang bersifat imunodominan, lestari, serta mewakili subtipe HCV yang bersirkulasi di Indonesia maupun global. Selanjutnya gen tersebut dibuat dengan teknik DNA sintetik oleh perusahaan penyedia jasa sintesis DNA dan diterima oleh peneliti dalam bentuk terklona pada plasmid pUC57. Untuk ekspresi pada sel Escherichia coli, gen penyandi antigen rekombinan disubklona dari plasmid pUC57 ke plasmid pQE80L dengan situs pengklonaan BamHI dan HindIII. Plasmid rekombinan hasil subklona kemudian dipropagasi pada sel Escherichia coli Top10 dan diverifikasi dengan teknik PCR koloni, analisis dengan enzim restriksi dan sekuensing. Gen penyandi antigen rekombinan HCV berbasis epitop multipel (HCV_ME) berukuran 1200 pb. Pengklonaan gen tersebut pada vektor pUC57 menghasilkan plasmid pUC57-HCV_ME (3910 pb) dan subklona pada vektor pQE80L menghasilkan plasmid pQE80L-HCV_ME (5909 pb). Berdasarkan pada hasil verifikasi plasmid pQE80L-HCV_ME pengekspresi antigen rekombinan untuk deteksi antibodi anti-HCV telah berhasil dikonstruksi.
The transmission of severe acute respiratory syndrome coronavirus 2 (SARS CoV-2) in Indonesia is seen to be uncontrollably increasing that urges the government to leverage the capacity for the disease detections. Real-time polymerase chain reaction (RT-PCR), rapid test and computed tomography (CT) scan are the most common methods to determine if one has been infected regardless of whether or not the common symptoms of such Corona Virus Disease 2019 (COVID-19) surface. Among these three, RT-PCR is considered the gold standard for qualitative and quantitative assessment of SARS CoV-2 detection. The present paper aims at elaborating the framework of Roche’s RT-PCR machine employed specifically for SARS CoV-2 detection performed by Genetics Indonesia which is deemed to be efficient and relatively quicker than other detection kits. RT-PCR machine detected SARS Cov-2 with RNA amplification curve equals to 10 copies RNA below the cut off value of Crossing point (Cp) positive control. Also elucidated in the paper is the implementations of EAV RNA and LightCycler® 96 RT-PCR System through which analysis time, amounts of individual required sample, as well as the reagents, can be accordingly reduced.
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