Present research discovered novel miRNA-SSRs for seed type trait from 761 potential precursor miRNA sequences of pomegranate. SSR mining and BLASTx of the unique sequences identified 69 non-coding pre-miRNA sequences, which were then searched for BLASTn homology against Dabenzi genome. Sixty three true pri-miRNA contigs encoding 213 pre-miRNAs were predicted. Analysis of the resulting sequences enabled discovery of SSRs within pri-miRNA (227) and pre-miRNA sequences (79). A total of 132 miRNA-SSRs were developed for seed type trait from 63 true pri-miRNAs, of which 46 were specific to pre-miRNAs. Through ePCR, 123 primers were validated and mapped on eight Tunisia chromosomes. Further, 80 SSRs producing specific amplicons were ePCR-confirmed on multiple genomes i.e. Dabenzi, Taishanhong, AG2017 and Tunisia, yielding a set of 63 polymorphic SSRs (polymorphism information content ≥0.5). Of these, 32 miRNA-SSRs revealed higher polymorphism level (89.29%) when assayed on six pomegranate genotypes. Furthermore, target prediction and network analysis suggested a possible association of miRNA-SSRs i.e. miRNA_SH_SSR69, miRNA_SH_SSR36, miRNA_SH_SSR103, miRNA_SH_SSR35 and miRNA_SH_SSR53 with seed type trait. These miRNA-SSRs would serve as important genomic resource for rapid and targeted improvement of seed type trait of pomegranate.
Variability studies of physico-chemical properties of pomegranate (Punica granatum L.) using a scoring technique. Abstract-Introduction. India is the largest producer of pomegranate in the world, with rich genetic diversity. The wide range of utility of pomegranate in human health, nutrition and livelihood security has triggered its heavy demand in India and other countries. At present, more than 300 germplasm accessions from indigenous and exotic sources are available in India, but meagre information on the physico-chemical properties of their fruits is available. In our study, the physico-chemical properties of varieties grown under a semi-arid climate were investigated. Materials and methods. Our study was carried out at the experimental farm of the National Research Centre on Pomegranate, Solapur, India, during the harvests of 2008-2009 and 2009-2010. Twenty pomegranate varieties were studied with respect to the physico-chemical properties of their fruits. Results and discussion. A considerable variation (coefficient of variation: 3.37% to 101.13%) was observed with respect to the 16 characters studied. The values ranged from 12.64°Brix to 15.39°Brix for TSS; 0.38% to 3.40% for acidity; 16.67 g to 27.82 g for 100-aril weight; 4.31-38.62 for the maturity index; 59.22% to 77.40% for aril percentage; and 43.30% to 61.00% for juice percentage. Fruit size, rind weight, total aril weight, 100-aril weight, juice weight and aril width were found to have a strong positive correlation (P < 0.01) with fruit weight. Based on the total index score obtained, the varieties 'Bassein Seedless', 'Dholka', 'Ganesh', 'Jyoti', 'Kandhari', 'KRS', 'Mridula' and 'P-13' were found to be suitable for processing and table purposes. India / Punica granatum / genetic resources / fruits / physico-chemical properties / genetic variation / selection index Étude de la variabilité de propriétés physico-chimiques de grenades (Punica granatum L.) par une technique de notation. Résumé-Introduction. L'Inde est le plus grand producteur de grenades dans le monde ; l'espèce présente une vaste diversité génétique dans ce pays. Un large éventail d'utilisation de la grenade pour la santé humaine et la sécurité alimentaire a déclenché une forte demande en Inde et ailleurs. À l'heure actuelle, plus de 300 accessions de matériel génétique d'origine locale ou exotique sont disponibles en Inde, mais peu d'informations sur les propriétés physico-chimiques de leurs fruits sont disponibles. Dans notre étude, les propriétés physico-chimiques de certaines variétés cultivées sous climat semi-aride ont été étudiées. Matériel et méthodes. Notre étude a été réalisée à la ferme expérimentale du Centre national de recherche sur la grenade (Solapur, Inde) pendant les campagnes de récolte de 2008-2009 et 2009-2010. Vingt variétés de grenadiers ont été étudiées quant aux propriétés physico-chimiques de leurs fruits. Résultats et discussion. Une variation considérable (coefficient de variation : 3,37 % à 101,13 %) a été observée pour 16 caractères étudiés. Les valeurs ont...
Pomegranate ( Punica granatum L.) is an important fruit crop, rich in fiber, vitamins, antioxidants, minerals and source of different biologically active compounds. The bacterial blight caused by Xanthomonas axonopodis pv. punicae is a serious threat to the crop leading to 60–80% yield loss under epiphytotic conditions. In this work, we have generated comparative transcriptome profile to mark the gene expression signatures during resistance and susceptible interactions. We analyzed leaf and fruits samples of moderately resistant genotype (IC 524207) and susceptible variety (Bhagawa) of pomegranate at three progressive infection stages upon inoculation with the pathogen. RNA-Seq with the Illumina HiSeq 2500 platform revealed 1,88,337 non-redundant (nr) transcript sequences from raw sequencing data, for a total of 34,626 unigenes with size >2 kb. Moreover, 85.3% unigenes were annotated in at least one of the seven databases examined. Comparative analysis of gene-expression signatures in resistant and susceptible varieties showed that the genes known to be involved in defense mechanism in plants were up-regulated in resistant variety. Gene Ontology (GO) analysis successfully annotated 90,485 pomegranate unigenes, of which 68,464 were assigned to biological, 78,107 unigenes molecular function and 44,414 to cellular components. Significantly enriched GO terms in DEGs were related to oxidations reduction biological process, protein binding and oxidoreductase activity. This transcriptome data on pomegranate could help in understanding resistance and susceptibility nature of cultivars and further detailed fine mapping and functional validation of identified candidate gene would provide scope for resistance breeding programme in pomegranate.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.