AbstractNatural polymers are being investigated with renewed exuberance as they have a tremendous unexploited potential. During the past few decades, much interest has developed in the biopolymer-based materials due to their biodegradable, nontoxic, biocompatible and non-allergic nature. Chitosan (CS) is the second most abundant naturally occurring amino polysaccharide after cellulose and is extracted from the shells of sea crustaceans. The primary amine group in CS is responsible for its various properties and it is derived from the deacetylated form of chitin. Its biocompatible, nontoxic, biodegradable and antimicrobial properties have led to significant research towards biomedical applications, such as tissue engineering, wound healing, drug delivery, obesity treatment, etc. This review summarizes the present work done by researchers in prospects of CS and its numerous applications in the biomedical field.
In the present study effect of chloroform fraction of Cassia fistula fruit pulp on cyto-morphological parameters like mycelium width and conidial size of Alternaria solani has been studied. Column chromatography of chloroform extract and TLC fingerprinting of column fractions were also done. Column fractions were screened for antifungal activity and fraction showing best activity was further subjected to GC MS analysis for the purification and identification of the structure of active compound. Result suggested that mycelium width of Alternaria solani increased up to 77.89% and conidia size of the was reduced up to 97.61% at 1.25 mg/ml (Sub MIC) concentration of the chloroform extract. Eight fractions obtained from column chromatography and fraction no. 2 (FPF-2) showed maximum inhibition i.e. 98.25% against Alternaria solani. Rf values of TLC bands of column fractions were found between the range from 0.60 to 0.97cm. GC-MS analysis reveals the presence of butanoic acid, 2-methyl-, Penthiophane (2H-Thiopyran, tetrahydro) and Isopropyl acetate (Acetic acid, 1-methyl ethyl ester). These three compounds are responsible for the antimicrobial activity of Cassia fistula fruit pulp.
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