FinlandTo investigate the intestinal immune responses during a diagnostic milk ~rovocation in p;llicnu (mean age 13.5 monw) with cow's milk dlFrgy (CMA), loWI &munoglobul~n-secrctmn cells (ISCs) and swclrc andbalv-sccrcun~ cells (ASCs) from wrlrrhcnl blood samples-were assessed by ELSA plaque & a y ( E~l b O~) .~~i f & n pa tic^^ had acute urticaria1 skin eruptions. 8 plicnu had slow onsel of eczema, and IS patients showed symptoms from Iho gasminleslinal uacL A significant increase in IgM secreting cells (geomeuic means with 195 % conlidence intervalsl) from 382.2 1265.552) lo 621.4 1381, 1013v10~ cells: t = 2.87.p < 0.01 but not IgA-and IgG-wreting cells was aswriated with acute urticaria. In patienu with ecmatous skin eruptions and gastrointestinal symptoms the response involved all these immunoglobulin isotypes. Altered immune status as evidenced by inappropriate immu~le reactions to liver membrane antigens occurs in children with CF and CLD, who also have an increased frequency of the "autoimmune" haplotypc HLA B8-DR3. To determine whether immune alterations typical of other autoimmune liver disease were present we investigated 143 children with CF, including 25 with CLD. Immunoglobulin Icvcls were measurcd by ncphelomctry and autoantibodies (AA) Lo nuclear (ANA), nucleolar (ANoA), mitochrondrial (AMA), smooth muscle (SLIA), gastric parietal cell (GPC) and liver, kidney microsomal (LKM) antigens by indirect immunolluorescrncc using rat kidney, livcr and gut and HEp-2 cells as subatrate.IgG and IgA wcre significantly highcr in children with LD thdn in those without (p<0.005 and <0.007 respectively) whereas IgM lcvcls were similar. AA were found in 96 (67%) childrcn (tilrcs 1:10 to 1:40, median 1:lO) : ANA in 56 (39%); ANoA in 55 (38%); SMA in 21 (15%); GPC in 13 (9%); AMA in 2 (1.31); LKM in 1 (1%).Tbc frcquoncy of AA was similar in children with and without LD but occurred in <2% of normal age matched controls.Humoral immunc abnormalities typical of auto immune diseases arc frequent in CF.Their pathophysiological role in CF is unclear. AA including a high and previously unrcported incidence of ANoA may arise from recurrent stirnulalion of the immune system by infection and/or tissue disruption but are not related to LD. High IgG and IgA in CF with CLD may derive from defective clearance by the liver. The development o r rat intestinal L-Ph specific activity displays a wall-known post weaning decline. In contrasl, total lactase activity increases to reach maximal levels around weaning. and remains high subsequently. In order to elucidate the molecular basis for theso patterns. a rat Iactsso cDNA was isolated and characterized, and used in the quantification of lactrue mRNA during development. This lactase cDNA uniquely hybridized to a 6.8 kb mRNA in the amall intestine. To assess the amount of lactare mRNA encoding for lactwe enzyme activity in the small intestine, total intestinal RNA was isolated and analyzed by Northern and dot blot hybridizntion. The pattern of total lactase mRNA during development follo...
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.