Plastics debris is accumulating in the environment and is fragmenting into smaller pieces; as it does, the potential for ingestion by animals increases. The consequences of macroplastic debris for wildlife are well documented, however the impacts of microplastic (< 1 mm) are poorly understood. The mussel, Mytilus edulis, was used to investigate ingestion, translocation, and accumulation of this debris. Initial experiments showed that upon ingestion, microplastic accumulated in the gut. Mussels were subsequently exposed to treatments containing seawater and microplastic (3.0 or 9.6 microm). After transfer to clean conditions, microplastic was tracked in the hemolymph. Particles translocated from the gut to the circulatory system within 3 days and persisted for over 48 days. Abundance of microplastic was greatest after 12 days and declined thereafter. Smaller particles were more abundant than larger particles and our data indicate as plastic fragments into smaller particles, the potential for accumulation in the tissues of an organism increases. The short-term pulse exposure used here did not result in significant biological effects. However, plastics are exceedingly durable and so further work using a wider range of organisms, polymers, and periods of exposure will be required to establish the biological consequences of this debris.

The Great Barrier Reef Marine Park, an area almost the size of Japan, has a new network of no-take areas that significantly improves the protection of biodiversity. The new marine park zoning implements, in a quantitative manner, many of the theoretical design principles discussed in the literature. For example, the new network of no-take areas has at least 20% protection per "bioregion," minimum levels of protection for all known habitats and special or unique features, and minimum sizes for no-take areas of at least 10 or 20 km across at the smallest diameter. Overall, more than 33% of the Great Barrier Reef Marine Park is now in no-take areas (previously 4.5%). The steps taken leading to this outcome were to clarify to the interested public why the existing level of protection was inadequate; detail the conservation objectives of establishing new notake areas; work with relevant and independent experts to define, and contribute to, the best scientific process to deliver on the objectives; describe the biodiversity (e.g., map bioregions); define operational principles needed to achieve the objectives; invite community input on all of the above; gather and layer the data gathered in round-table discussions; report the degree of achievement of principles for various options of no-take areas; and determine how to address negative impacts. Some of the key success factors in this case have global relevance and include focusing initial communication on the problem to be addressed; applying * email leannef@gbrmpa.gov.au the precautionary principle; using independent experts; facilitating input to decision making; conducting extensive and participatory consultation; having an existing marine park that encompassed much of the ecosystem; having legislative power under federal law; developing high-level support; ensuring agency priority and ownership; and being able to address the issue of displaced fishers.Key Words: biophysical operational principles, cultural operational principles, economic operational principles, reserve-design software, social operational principles Establecimiento deÁreas sin Captura Representativas en la Gran Barrera Arrecifal: Implementación a Gran Escala de la Teoría sobreÁreas Marinas Protegidas Resumen: El Parque Marino Gran Barrera Arrecifal, con una superficie casi del tamaño de Japón, tiene una red deáreas sin captura que incrementa la protección de la biodiversidad significativamente. La nueva zonificación en el parque marino implementa, de manera cuantitativa, muchos de los principios teóricos de diseño discutidos en la literatura. Por ejemplo, la nueva red deáreas sin captura tiene niveles mínimos de protección de por lo menos 20% de protección por "bioregión" en todos los hábitats y rasgos especiales oúnicos conocidos, y tamaños mínimos para lasáreas sin captura de por lo menos 10 o 20 km en el diámetro menor. En general, más de 33% del Parque Marino Gran Barrera Arrecifal está enáreas sin captura (4.5% anteriormente). Los pasos hacia este resultado fueron clarificar al público interesa...

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