In the past decades it was noticed that there was a gradual increase in neoplasia diagnostics in animals, which are attributed to the longevity achieved by efficient vaccines, antibiotics, diagnostics, and food quality. The aliphatic oncology uses chemotherapics that are citostatic association, citostatic antibiotics, antimetabolic citostatics, and antimetabolic hormones in tumor treatment. Later on, the oncology introduced the imunomodulated antineoplasics from vegetals which belongs the viscum album. This study verified the efficiency of the extract of the injectable viscum album with concentrations D3 (0.1 μg/mL), D6 (0.1 ng/mL), D9 (0.1×10−3 ng/mL), D12 (0.1×10−6 ng/mL), and D30 (0.1×10−24 ng/mL) in culture of Caco-2 cells in vitro evaluated by correlating the spectral information obtained by dispersive Raman spectroscopy at 830 nm excitation with the cell viability determined by the Mitochondrial Activity (MTT) assay test. Results of MTT indicated that viscum album acted in the mitochondrial activity by reducing cellular viability in the Caco-2 cells in the concentrations of D3, D6, and D9. It was found that there was a good correlation of the Raman spectrum analyzed by principal components analysis, with the values of the MTT, and such result indicated that Raman could constitute in a sensitive tool for the detection of changes in the mitochondrial activity of Caco-2 cells.
The obtained results are reasonable according to classical biochemical analysis. Our proposed FT-Raman-based method was shown to be suitable for the monitoring of carbohydrate concentration in human blood, and presented some advantages over classical biochemical methods, such as real-time analysis, required small sample volume, and was nondestructive, and the samples did not need any previous treatment.
O envelhecimento e a obesidade representam fatores de risco bem conhecidos para que haja o desenvolvimento do câncer. Os tratamentos tem como objetivo a redução progressiva geral do tumor, um prolongamento do intervalo livre de doença e melhora na qualidade de vida através do alívio dos sinais associados ao câncer. O fato é que mesmo que cientificamente tenhamos evoluído muito nos últimos 10 anos através do conhecimento da Biologia do Cancer, os próximos anos serão de vital importância para melhorarmos a terapêutica, devido ao grande numero de casos, ainda estamos longe de erradicar o câncer. Esta revisão tem como objetivo proporcionar uma informação concisa ao medico veterinário, alertando e esclarecendo as diversas formas de Prevenção na fase pré-miasma segundo Hahnemann, utilizando conhecimento das doenças crônicas e associando as novas possibilidades Terapêuticas e de Diagnostico. Concluímos que não são suficientes tratamentos e sistemas diagnósticos sofisticados que não mudaram as estatísticas do câncer, é necessário traçar programas de prevenção contínua e precoce mesmo em pacientes cirúrgicos.
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