1. An 870-fold purification of glucokinase from rat liver is described which involves ammonium sulphate fractionation and the use of DEAE-Sephadex, DEAE-cellulose and polyacrylamide columns. 2. The preparation is free of any interfering enzymes and has a specific activity of 8mumoles/min./mg. of protein. 3. Glucokinase catalyses the phosphorylation of glucose, mannose and 2-deoxyglucose. 4. The enzyme is inhibited by high concentrations of glucose 6-phosphate only; ADP is an inhibitor whose effect depends on the Mg(2+) concentration. 5. The properties of glucokinase are compared briefly with those of other phosphotransferases.
Scientific monitoring of river health is well established and has a significant role to play in environmental assessment by communities, managers and policy makers. Cultural indicators help to articulate cultural values, assess the state of the environment from a cultural perspective and assist with establishing a role for Māori in environmental monitoring. We reviewed the philosophies behind cultural and scientific monitoring of river health and compared the results from the two approaches at 25 sites in the Motueka and Riwaka catchments. Both scientific and cultural indicators suggested a decrease in river health in relation to increased land-use pressure. There were also correlations between the results from the two approaches suggesting cultural indicators could be used in a similar manner as scientific indicators to set environmental benchmarks. Using scientific approaches alongside culturally based monitoring provides a wealth of knowledge to understand better what we mean by river health. The two approaches can be regarded as complementary and reflect two different knowledge systems and perspectives.
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