Analysis of cardiac troponin I (cTn I) and T (cTnT) are considered the "gold standard" for the non-invasive diagnosis of myocardial injury in human and animals. It has replaced traditionally used cardiac biomarkers such as myoglobin, lactate dehydrogenase (LDH), creatine kinase (CK) and CK-MB due to its high sensitivity and specificity for the detection of myocardial injury. Cardiac troponins are proteins that control the calcium-mediated interaction between actin and myosin, allowing contraction at the sarcomere level. Concentration of the cTn can be correlated microscopic lesion and loss of immunolabeling in myocardium damage. Troponin concentration remains elevated in blood for 1-2wks so that wide window is available for diagnosis of myocardial damage. The cTn test has >95% specificity and sensitivity and test is less time consuming (10 to 15 minutes) and less costly (INR 200 to INR 500).
Aim: An experiment was conducted to study the haemato-biochemical alterations induced by Diclofenac Sodium toxicity. Materials and Methods: 48 Swiss albino mice of either sex, divided uniformly into four different groups. The mice of group I received only deionised water as control while, group II, III and IV were given Diclofenac sodium @ 2.37 mg/kg B.W, 4.75 mg/kg B.W, 9.5 mg/kg B.W orally for 28 days. Results: In dose dependant significant reductions in TEC, Hb, PCV, MCV, MCHC were observed. No significant change was observed in total WBC count in both the sexes. However, relative values of leukocytes indicated relative neutrophilia and relative lymphopenia in higher group. Biochemically dose dependant significant changes were observed for AST, ALT, Total bilirubin, Total protein, Albumin, Globulin, Cholesterol, Urea, Creatinine and Uric acid in male and female animals. Conclusion:The present study indicates hepatobilliary, nephric and gastrointestinal toxicity in albino swiss mice due to Diclofenac Sodium Toxicity.
Squamous cell carcinoma (SCC) of horn is frequently observed in Bos indicus affecting 1% of cattle population and accounting 83.34% of total tumours found. The transcriptome profile of horn cancer (HC) tissue and the matched normal (HN) tissue were analysed by RNA-seq using Roche 454 sequencing. A total of 1 504 900 reads comprising of 612 MB data were used to identify differentially expressed genes using CLC Genomic Workbench. These include up-regulation of KRT6A, KRT6B, KRT6C, KRT14, SFN, KRT84, PI3, COL17A1, ANLN, SERPINB5 and down-regulation of BOLA, SCGB1A1, CXCL17, KRT19, BPIFB1, NR4A1 and TFF3 in HC, which are involved in regulation of gene transcription, cell proliferation, apoptosis, cell survival and metabolic pathways. The qPCR analysis of several targets suggested concordance of gene expression profile with RNA-seq analysis. The present findings would provide basis for further screening of genes and identification of markers for early diagnosis and therapeutic intervention of HC.
Background Caprine skin is a promising biomaterial for tissue‐engineering applications. However, tissue processing is required before its xenogenic use. Aims Therefore, the purpose of this study was to evaluate the structural integrity and biocompatibility of the caprine skin after de‐epithelialization, using sodium chloride (NaCl) and trypsin solutions, followed by de‐cellularization using sodium dodecyl sulfate (SDS) solution. Materials & Methods The caprine skin was de‐epithelialized using NaCl (2‐4 mol/L) and trypsin (0.25%‐0.5%) followed by the treatment of SDS (1%‐4%) solution over a period of time. Acellularity of the prepared matrix was confirmed histologically and characterized by appropriate staining, scanning electron microscopy (SEM), DNA quantification, and Fourier‐transform infrared (FTIR) spectroscopy. The caprine acellular dermal matrix (CADM) was used for the repair of spontaneously occurring abdominal hernia in ten buffaloes. The biocompatibility of the CADM was evaluated using clinical, hematological, biochemical, and anti‐oxidant parameters. Results Histologically, the skin treated with 0.25% trypsin in 4 mol/L NaCl for 8 hours resulted in complete de‐epithelialization. Further treatment with 2% SDS for 48 hours demonstrated complete acellularity and orderly arranged collagen fibers. The SEM confirmed a preservation of collagen arrangement within CADM. The DNA content was significantly (P < .05) lower in CADM (46.20 ± 7.94 ng/mg) as compared to fresh skin (662.56 ± 156.11 ng/mg) indicating effective acellularity. The FTIR spectra showed characteristic collagen peaks of amide A, amide B, amide I, amide II, and amide III in CADM. All the 10 animals recovered uneventfully and remained sound. Hematological, biochemical, and anti‐oxidants findings were unremarkable. Conclusion Results indicated the acceptance and biocompatibility of the xenogenic caprine acellular dermal matrix for abdominal hernia repair in buffaloes without complications.
Treatment with 2% SDS for 48 h results in 92.54% reduction in DNA contents and complete acellularity of the bubaline diaphragm.• FTIR spectrum of decellularized diaphragm has shown all characteristic transmittance peaks of the collagen.• Bubaline diaphragm matrix shows excellent repair efficiency and biocompatibility for abdominal hernia repair in cattle without complications.• Insert a highlight no longer than 85 characters. HIGHLIGHTS 2 Vora, S.D.; et al.
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