The present study was planned to investigate significant polymorphism in exon3 and promoter regions of prolactin gene in Gaolao cattle and its association (if any) with milk components. A total of 50 randomly selected Gaolao cows were genotyped for PRLGI-RsaI and PRLGIII-Tsp45I loci using PCR-RFLP technique. The PRLGI-RsaI locus was polymorphic and PRLGIII-Tsp45I locus was monomorphic indicating genetic variation at PRLGI-RsaI locus in Gaolao cattle. The allele frequency for the PRLA alleles was found 0.933 and for allele PRLB was 0.067 at PRLGI–RsaI locus. The result of analysis variance showed significant (PÂ0.05) effect of AA genotype on fat % as compared to AB genotype; however non-significant effect of these genotypes was found on protein %, SNF % and lactose % which may be due to small sample resulting in higher error variance. The results of present study indicated that identified prolactin gene polymorphism at PRLGI-RsaI in exon 3 after validation in large breeding populations having precise data records may prove as important candidate gene marker for selection and breeding decisions in genetic improvement of indigenous cattle for milk productivity.
The experiment was undertaken collaboratively at the Department of Animal Genetics and Breeding, Nagpur Veterinary College, Nagpur; National Environmental Engineering Research Institute (NEERI), Nagpur to study the mutagenic effect of fly ash on bovine Amino Levulinic Acid Dehydratase (ALAD) gene. DNA was extracted from blood samples of 49 fly ash exposed cattle born and reared in vicinity of two Thermal Power Plants, Koradi and Khaperkheda and 41 healthy animals reared at organised farms located more than 10 kilometres away from the plants as the control group. Polymerase Chain Reaction and DNA sequencing of the PCR products were carried out for mutation analysis. 'MEGA-5, Bioedit and 'Clustal Omega' software were used to analyse the sequenced data. Thirteen animals from fly ash exposed group revealed mutations at different loci. Most of the animals exhibiting mutations had phenotypic expression of heavy metal toxicity. The nucleotide substitution variant G C was observed in highest frequency. Mutations in bovine ALAD gene may be due to a prolonged exposure to heavy metals and other toxic elements present in fly ash released from the thermal power plants. Further, it serves as ALAD gene may be used as a good candidate for further toxico-genomic studies.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.