An understanding of the genetic diversity and structure of plant species is essential in order to comprehend the degree of biodiversity loss and to develop successful restoration programs. Handroanthus is an important genus that presents one of the most valuable timbers of South America. Handroanthus chrysanthus is an important species distributed in Central and South America. Microsatellite markers are not previously developed for this species. Ten microsatellites for Handroanthus chrysanthus developed using high-throughput sequencing are presented here. The usefulness of these microsatellite loci for the genetic analysis of subspecies H. chrysanthus subsp. chrysanthus (distributed in coastal dry forests) and subspecies H. chrysanthus subsp. meridionalis (distributed in premontane moist forests) is analyzed. At least eight polymorphic microsatellites are useful for each subspecies, seven of which can be used in both subspecies.
3D printing in industries like medicine and education has led to major
improvements in designing, developing, and printing useful models, including
body prostheses and educational toys. The 3D printer's commercialization has
risen thanks to more manufacturers, models, and reasonably priced equipment.
As a result, prototypes and gadget creation have merged cutting-edge design
techniques like gamification and electronic components, allowing
accessibility and interactive and enjoyable devices. This paper describes
the design, production, implementation, operation, codification, and
printing of a 3D prototype of an interactive adding machine to improve basic
numerical skills and help children and others with cognitive disabilities
learn easier or lessen distractions. The prototype and all the necessary
documentation are available for anyone to replicate. The paper also
discusses difficulties encountered during the design process and proposes
future improvements for the next version.
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