The anti-oxidative enzyme, glutathione peroxidase 4 (GPX4), helps to promote inflammation resolution by eliminating oxidative species produced by the arachidonic acid (AA) metabolic network. Up-regulating its activity has been proposed as a promising strategy for inflammation intervention. In the present study, we aimed to study the effect of GPX4 activator on the AA metabolic network and inflammation related pathways. Using combined computational and experimental screen, we identified a novel compound that can activate the enzyme activity of GPX4 by more than two folds. We further assessed its potential in a series of cellular assays where GPX4 was demonstrated to play a regulatory role. We are able to show that GPX4 activation suppressed inflammatory conditions such as oxidation of AA and NF-κB pathway activation. We further demonstrated that this GPX4 activator can decrease the intracellular ROS level and suppress ferroptosis. Our study suggests that GPX4 activators can be developed as anti-inflammatory or cyto-protective agent in lipid-peroxidation-mediated diseases.

In medium-/high-power inverters, optimal pulsewidth modulation (OPWM) is often used to reduce the switching frequency and at the same time, realize selective harmonic elimination (SHE). For both two-level and multilevel inverters, most selective harmonic elimination (SHE) studies are based on solving multiple variable high-order nonlinear equations. Furthermore, for multilevel inverters, SHE has been often studied based on the assumption of balanced dc levels and single switching per level. In this paper, the authors further developed harmonics injection and equal area criteria-based four-equation method to realize OPWM for two-level inverters and multilevel inverters with unbalanced dc sources. For the cases, where only small number of voltage levels are available, weight oriented junction point distribution is utilized to enhance the performance of the four-equation method. A case study of multilevel inverter at low-modulation index is used as an example. Compared with existing methods, the proposed method does not involve complex equation groups and is much easier to be utilized in the case of large number of switching angles, or multiple switching angles per voltage level in multilevel inverters.

This research study examined wildlife tourism experiences in a unique place and species combination; giant pandas at the Chengdu Research Base for Giant Panda Breeding (CRBGPB) in Sichuan, China. Content analysis was done on tourist reviews of CRBGPB on TripAdvisor.com in early 2012. The thematic analysis qualitative method was adopted in examining tourists' written reviews of experiences interacting with giant pandas. ROST CM6 and NVivo 8 were used for the content analysis and qualitative coding. A variety of tourist experiences, motivations, and preferences were revealed. Although tourists' experiences were varied, satisfaction increased with closer encounters and interactions with the giant pandas. The thematic analysis identified three features of the wildlife tourism experiences at CRBGPB; tourists, giant pandas, and settings. Implications and management recommendations were identified for places offering wildlife tourism experiences.

Background & Aims The outbreak of coronavirus disease 2019 (COVID‐19) has been declared a pandemic. Although COVID‐19 is caused by infection in the respiratory tract, extrapulmonary manifestations including dysregulation of the immune system and hepatic injury have been observed. Given the high prevalence of hepatitis B virus (HBV) infection in China, we sought to study the impact of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) and HBV coinfection in patients. Methods Blood samples of 50 SARS‐CoV‐2 and HBV coinfected patients, 56 SARS‐CoV‐2 mono‐infected patients, 57 HBeAg‐negative chronic HBV patient controls and 57 healthy controls admitted to Renmin Hospital of Wuhan University were collected in this study. Complete blood count and serum biochemistry panels including markers indicative of liver functions were performed. Cytokines including IFN‐γ, TNF‐α, IL‐2, IL‐4, IL‐6 and IL‐10 were evaluated. T cell, B cell and NK cell counts were measured using flow cytometry. Results SARS‐CoV‐2 and HBV coinfection did not significantly affect the outcome of the COVID‐19. However, at the onset of COVID‐19, SARS‐CoV‐2 and HBV coinfected patients showed more severe monocytopenia and thrombocytopenia as well as more disturbed hepatic function in albumin production and lipid metabolism. Most of the disarrangement could be reversed after recovery from COVID‐19. Conclusions While chronic HBV infection did not predispose COVID‐19 patients to more severe outcomes, our data suggest SARS‐CoV‐2 and HBV coinfection poses a higher extent of dysregulation of host functions at the onset of COVID‐19. Thus, caution needs to be taken with the management of SARS‐CoV‐2 and HBV coinfected patients.