Cumulative data suggest that neuroinflammation plays a prominent role in Alzheimer's disease (AD) pathogenesis. The purpose of this work was to assess if patients with AD present a specific cerebrospinal fluid (CSF) cytokine profile and if it correlates to disease progression. We determined the levels of 27 cytokines in CSF of patients with AD and compared them with patients with frontotemporal dementia and nondemented controls. In addition, we correlated the cytokine levels with cognitive status and disease progression after 12 months. Patients with AD had higher levels of proinflammatory and anti-inflammatory cytokines (eotaxin, interleukin [IL]-1ra, IL-4, IL-7, IL-8, IL-9, IL-10, IL-15, granulocyte colony-stimulating factor, monocyte chemotactic protein 1, platelet-derived growth factor, tumor necrosis factor alfa) compared to nondemented controls. There was a negative correlation between the disease progression and the levels of several cytokines (IL-1b, IL-4, IL-6, IL-9, IL-17A, basic fibroblast growth factor, granulocyte colony-stimulating factor, granulocyte-macrophage colony-stimulating factor, interferon gamma, macrophage inflammatory proteins-1b). To the best of our knowledge, this is the first study reporting a "protective" role of the upregulation of specific intrathecal cytokine levels in AD. This finding supports that a fine "rebalancing" of the immune system represents a new target in AD therapeutic approach.

The main goal of this study was to produce adjusted normative data for the Portuguese population on two verbal fluency measures: the semantic fluency test (animals category) and the phonemic fluency test (letters M, R, and P). The study included 950 community-dwelling individuals (624 women and 326 men) aged between 18 and 98 (mean = 57.8, SD = 19.0), who had educational backgrounds ranging from 0 to 20 years (mean = 8.8, SD = 5.2). The results showed that age and education were significantly associated with semantic fluency and phonemic fluency performance. These demographic characteristics accounted for 42% of the semantic fluency and between 23% and 31% of the phonemic fluency performance variance. No significant sex effects were found. The normative data are presented as regression-based algorithms to adjust test scores for age and education, with subsequent correspondence between adjusted scores and percentile distribution.

The main goal of this study was to produce normative data for the Portuguese population on the Trail Making Test (TMT). The study included 1,038 community-dwelling individuals aged between 18 and 93 years, who had educational backgrounds ranging from 3 to 22 years. The results showed that sex, age, and education were significantly associated with TMT performance. These demographic characteristics accounted for 57% of the performance variance at part A and 50% at part B. The normative data are presented as regression-based algorithms to adjust direct and derived test scores for sex, age, and education. The adjusted scores' percentile distributions and their correspondence with scaled scores are also provided.