No abstract

To study the effect of nitrate (NO3(-)) on selenate (SeO4(2-)) reduction, we tested a H2-based biofilm with a range of influent NO3(-) loadings. When SeO4(2-) was the only electron acceptor (stage 1), 40% of the influent SeO4(2-) was reduced to insoluble elemental selenium (Se(0)). SeO4(2-) reduction was dramatically inhibited when NO3(-) was added at a surface loading larger than 1.14 g of N m(-2) day(-1), when H2 delivery became limiting and only 80% of the input NO3(-) was reduced (stage 2). In stage 3, when NO3(-) was again removed from the influent, SeO4(2-) reduction was re-established and increased to 60% conversion to Se(0). SeO4(2-) reduction remained stable at 60% in stages 4 and 5, when the NO3(-) surface loading was re-introduced at ≤ 0.53 g of N m(-2) day(-1), allowing for complete NO3(-) reduction. The selenate-reducing microbial community was significantly reshaped by the high NO3(-) surface loading in stage 2, and it remained stable through stages 3-5. In particular, the abundance of α-Proteobacteria decreased from 30% in stage 1 to less than 10% of total bacteria in stage 2. β-Proteobacteria, which represented about 55% of total bacteria in the biofilm in stage 1, increased to more than 90% of phylotypes in stage 2. Hydrogenophaga, an autotrophic denitrifier, was positively correlated with NO3(-) flux. Thus, introducing a NO3(-) loading high enough to cause H2 limitation and suppress SeO4(2-) reduction had a long-lasting effect on the microbial community structure, which was confirmed by principal coordinate analysis, although SeO4(2-) reduction remained intact.

Chromate (Cr­(VI)), as one of ubiquitous contaminants in groundwater, has posed a major threat to public health and ecological environment. Although various electron donors (e.g., organic carbon, hydrogen, and methane) have been proposed to drive chromate removal from contaminated water, little is known for microbial chromate reduction coupled to elemental sulfur (S(0)) or zerovalent iron (Fe(0)) oxidation. This study demonstrated chromate could be biologically reduced by using S(0) or Fe(0) as inorganic electron donor. After 60-day cultivation, the sludge achieved a high Cr­(VI) removal efficiency of 92.9 ± 1.1% and 98.1 ± 1.2% in two independent systems with S(0) or Fe(0) as the sole electron donor, respectively. The deposited Cr­(III) was identified as the main reduction product based on X-ray photoelectron spectroscopy. High-throughput 16S rRNA gene sequencing indicated that Cr­(VI) reduction coupled to S(0) or Fe(0) oxidation was mediated synergically by a microbial consortia. In such the consortia, S(0)- or Fe(0)-oxidizing bacteria (e.g., Thiobacillus or Ferrovibrio) could generate volatile fatty acids as metabolites, which were further utilized by chromate-reducing bacteria (e.g., Geobacter or Desulfovibrio) to reduce chromate. Our findings advance our understanding on microbial chromate reduction supported by solid electron donors and also offer a promising process for groundwater remediation.

The influence of multi-walled carbon nanotubes (MWCNT) on the structural dynamic behavior of MWCNT/epoxy nanocomposites was investigated. Two different types of MWCNTs, pristine MWCNT and functionalized MWCNT, were used in this study. Carboxylic acid-functionalized MWCNTs (MWCNT-COOH) were obtained by oxidation pristine MWCNTs via sonication in sulfuric-nitric acid and characterized by Fourier transform infrared spectroscopy (FTIR). Dynamic behaviors of the MWCNT reinforced nanocomposite including the natural frequency and damping ratio were determined using free vibration test. Experimental results showed that the damping ratio of the nanocomposite decreases with the increase of the MWCNT addition, while the natural frequency is increasing with the increase of the MWCNT addition. Functionalized MWCNTs improved the interfacial bonding between the nanotubes and epoxy resin resulting in the reduction of the interfacial energy dissipation ability and enhancement of the stiffness.

Reduction in methane emissions to the Earth’s atmosphere is a critical strategy for tackling climate change. It is well established that anaerobic oxidation of methane (AOM) associated with sulfate reduction...