Cervical cancer is one of the most common cancers in women living in developing countries. Due to a lack of affordable effective therapy, research into alternative anticancer compounds with low toxicity such as dietary polyphenols has continued. Our aim is to determine whether two structurally similar plant polyphenols, resveratrol and pterostilbene, exhibit anticancer and anti-HPV (Human papillomavirus) activity against cervical cancer cells. To determine anticancer activity, extensive in vitro analyses were performed. Anti-HPV activity, through measuring E6 protein levels, subsequent downstream p53 effects, and caspase-3 activation, were studied to understand a possible mechanism of action. Both polyphenols are effective agents in targeting cervical cancer cells, having low IC50 values in the µM range. They decrease clonogenic survival, reduce cell migration, arrest cells at the S-phase, and reduce the number of mitotic cells. These findings were significant, with pterostilbene often being more effective than resveratrol. Resveratrol and to a greater extent pterostilbene downregulates the HPV oncoprotein E6, induces caspase-3 activation, and upregulates p53 protein levels. Results point to a mechanism that may involve the downregulation of the HPV E6 oncoprotein, activation of apoptotic pathways, and re-establishment of functional p53 protein, with pterostilbene showing greater efficacy than resveratrol.
Buildings in Canada account for a significant amount of greenhouse gas (GHG) emissions and net zero energy building technology has been identified as part of the solution. This study presents a conceptual model identifying barriers to the adoption of net zero energy housing and tests it by administering a survey to 271 participants in a net zero energy housing demonstration project in Toronto, Canada. Using multivariate correlation and multi-linear regression analyses this study finds that of all the innovation adoption variables it was the construction and design quality that was the most significant contributor to the adoption of a net zero energy home by a potential home owner. This study found that the (a) extra cost compared to a conventional home, b) lack of knowledge about the technology associated with a net zero energy home or (c) not knowing someone who owned a net zero energy home were not significant barriers to accepting net zero energy homes. Our results suggest that policy-makers should promote the diffusion of net zero energy home technology by encouraging housing developers to include net zero energy homes in their collection of model homes, with an emphasis on quality design and construction. Furthermore, engaging in trust building initiatives such as education and knowledge about the technology, its related energy cost savings, and the environmental benefits would contribute to a greater acceptance of net zero energy homes.
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