The yV-glycosidic saccharide chains of S-glycoproteins associated with self-incompatibility in Brassica campestris were converted to the pyridylamino derivatives, and their structures were determined with physicochemical data and with the exoglycosidase treatment. These proved to be related to a saccharide chain of pineapple stem bromelain. The three iS-glycoproteins, S8, S9 and Sx 2, carried the commonoligosaccharides A and B. This indicates that the specificity of tS-glycoproteins was not present in the saccharide chains themselves, but probably in the amino acid sequences.Self-incompatibility in higher plants is designated as the "inability of a fertile hermaphrodite seed-plant to produce zygotes after self pollination," and has been observed in many angiosperm species.1* Self-incompatibility in the cruciferous plant, Brassica, is under the sporophytic control of S-locus. Until now, over 50 S-alleles have been identified and the presence of many S-locus-specific glycopro-
Materials and MethodsPurification of S-glycoproteins. Three homozygous strains of B. campestris, S8S8, S9S9 and S12S12, were cultivated in a greenhouseand the stigmas were collected on their flowering days. Extraction and purification of the S-glycoproteins were performed by the methods previously reported.2<5) Analysis of the sugar composition of the S8-glycoprotein and pyridylamino derivatives of the saccharide chains. The S8-glycoprotein (50fig) was hydrolyzed with 2.5m trifluoroacetic acid (100 /il) at 100°C for 6 hr in an evacuated sealed tube. L-Rhamnose (2nmol) was added to the mixture as an internal standard, before the solution was passed through a small column ofAmberlite CG-120 (H+,
Flowers of many higher plants have a intraspecific recognition system between pollen and pistils, and this system controls self-incompatibility; the pollen whose phenotype is iden
Self-incompatibility in the cruciferous plant, Brassica, is strongly associated with glycoproteins, which are specific to S-locus and then termed S-glycoproteins; but their structures and functions are not yet known.1}In the preceding paper,2) we described the isolation, amino acid composition and N-terminal amino acid sequences of *S8, S9 and S12-glycoproteins from three different strains of B. campestris, and also reported that S8- appearing before 7 min were attributed to byproducts from reagents, and the peaks around 7.5min were mixtures of several minor saccharides and probably contained some byproducts of the major peaks.4) Therefore it was concluded that the carbohydrate chains of S8, S9 and S12-glycoproteins were commonly composedof two main substances, oligosaccharides A (la) and B (2a), which gave the
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