A fermentative acetic acid producing strain, named CSJ-3, was isolated from sludge and identified as Pseudomonas sp. on the basis of 16S rRNA gene analysis. The effects of cultivation conditions on the production of acetic acid by Pseudomonas sp. CSJ-3 were investigated in batch fermentation, and the maximum yield of acetic acid reached up to 0.49 % during 30 h cultivation under the optimum growth condition, including fermentation temperature of 37.0 °C, fermentation pH of 4.50, ethanol concentration of 8 %, carbon source (glucose) amount of 10 g/L, and rotation speed of 120 r/min. When ultraviolet (UV) irradiation was used for the mutation in culture medium to improve the yield of acetic acid, the results showed that the yield of acetic acid reached up to 0.59 %. UV and FTIR confirmed that acetic acid was the major fermented product.
This paper takes the double predriven recovery rooms (DPRR) of 31109 panel of a coal mine in Inner Mongolia as a case study. DPRRs are used to withdraw mining equipment, which play a significant role in safe and efficient production in the final longwall mining stage. Theoretical analysis and numerical simulation were carried out to study the reasonable size of the front abutment pillar between DPRR (inter-DPRR pillar) and the damage depth of the DPRR floor. The results show that (1) the stress distribution of the fender (the remnant longwall panel) can be approximately divided into three stages with the advance of the working face: stress redistribution (the first) stage, stress superimposed growth (the second) stage, and stress transfer (the third) stage. (2) According to stress distribution and the corresponding failure mode of the fender, the calculation model of the slippage damage of the DPRR floor is rectified, and the damage range of the floor is rezoned to make it more suitable for the damage depth of the room. (3) The zone of influence of the front abutment pressure is 40–50 m, and the stress around the DPRR increases significantly in the final mining stage. When the size of the inter-DPRR pillar is greater than 15 m, the effect of increasing the coal pillar size on lowering the peak stress of the main predriven recovery room is limited. (4) Floor heave tends to increase at first and then decrease with depth and reaches the maximum in the depth of 5 m in the final mining stage, indicating that 5 m is the starting point for the initial depth of the floor heave. (5) The theoretical calculation shows that the reasonable size of the inter-DPRR pillar is 20 m, and the critical width of the fender is 18.48 m, which can guide the secondary support to prevent dynamic disasters. Floor grouting and constructing concrete floor are effective and economic ways to control the floor heave.
Severe deformation and failure frequently occur in roadways with soft or weak surrounding rock and have greatly influenced safe and efficient mining of coal in many coal mines. Using portland cement, emery and fly ash as main raw materials, through laboratory tests, effect of water/binder ratio, cement/sand ratio, water/sodium silicate ratio, water reducing agent, fly ash/cement ratio and various performance indexes of grout of fluidity, viscosity, setting time, bleeding rate, compressive strength, concretion rate and various performance indexes were systematically analyzed. An optimized mixture ratio of the main raw materials added in the grouting material proportion was determined through uniform design method, an optimal mixture ratio was determined by regression analysis. The results show that: 1) The flow performance is significantly affected by change of sodium silicate and water reducer, the compressive strength of grouting material increases significantly with increase in emery content, and decreases significantly with increase in water reducer. 2) An optimized mixture ratio among water cement ratio, cement sand ratio, water/sodium silicate ratio, water reducing agent, fly ash/cement ratio in the grouting material is 0.75, 1.2, 8%, 3% and 0.18, respectively. Field test demonstrated that the material has better performance in reinforcing weak and broken rock mass.
Severe deformation and failure frequently occur in roadways with soft or weak surrounding rock and have greatly influenced safe and efficient mining of coal in many coal mines. Using portland cement, emery and fly ash as main raw materials, through laboratory tests, effect of water/binder ratio, cement/sand ratio, water/sodium silicate ratio, water reducing agent, fly ash/cement ratio and various performance indexes of grout of fluidity, viscosity, setting time, bleeding rate, compressive strength, concretion rate and various performance indexes were systematically analyzed. An optimized mixture ratio of the main raw materials added in the grouting material proportion was determined through uniform design method, an optimal mixture ratio was determined by regression analysis. The results show that: 1) The flow performance is significantly affected by change of sodium silicate and water reducer, the compressive strength of grouting material increases significantly with increase in emery content, and decreases significantly with increase in water reducer. 2) An optimized mixture ratio among water cement ratio, cement sand ratio, water/sodium silicate ratio, water reducing agent, fly ash/cement ratio in the grouting material is 0.75, 1.2, 8%, 3% and 0.18, respectively. Field test demonstrated that the material has better performance in reinforcing weak and broken rock mass.
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