S c w Y o r k Utiiversitg irwo P L m m (NINETEEN FIGURES)I n a previous paper (Dawson, '28) the possible identificat,ion, in the erythrocyt,e of Kcct'nrns, of t'he scgrogation apparatus with the Golgi apparatus was suggested. The same methods, t.he techiiic of Wright aiid Gicmsa., snpravital staining, the techiiic of ~assonov-T(ola.t.chev, and prolonged cxposnre to osmic acid iii a, hanging-drop, have been applied to the thrombocytcs arid leucocytes of Nectnrus. All of t.hcse several methods, however, were not applied with equal snccess to each of t,he six types of cells .under iiivcstigation. In an earlier study 011 t.he blood of the frog, Jordan ('25), using supravit.al staining with neutral red, dcmonst.ratcd the presence of a. segrega.tion apparatus of granules arid globules in cve.rp t.ppe of blood cell of t.his animal. The present st.udy of the blood of Ncctnrus also discloses the presence of a similar apparat,ns in practically every type of ccll. In Nectiirus, however, tlic elcmcnts of t.he segregation apparatus are not so geiicrally dist.ributed t.hroughout t>lie cytoplasm as in thc case of the frog, but usually are grouped and restric.t.ed. to certain areas of the different. cells.
MATERIAL AND M1Yl"l'IOT)SFresh l~lood, f o r smears, hanging-drop preparations, and snpravital staiiiing, ~v a s obtained directly from the braiiclii 211 circulation by the method described by Charipper ( '28) 335 TI[X %NATO>IIC\L REPORI), V O L . 41, NO. :j