OBJECTIVE To explore the relationship between the methylation status of the promoter 5'CpG island region and the biological behavior of human colorectal cancer RKO cells in vitro. METHODS RKO cells were treated with a selective DNA methyltransferase inhibitor 5-aza-2'-deoxycytidine (5-aza-CdR) for 72 h. Methylationspecifi c PCR (MSP), T-A cloning and DNA sequence analysis were used to determinate the 5'CpG island methylation status of the p16/CDKN2 tumor suppressor gene. Cell growth, morphological changes and apoptosis were analyzed by the MTT assay, fl ow cytometry, fl uorescence staining and electron microscopy. RESULTS The 5'CpG island of the p16/CDKN2 tumor suppressor gene in RKO cells was a typically hypermethylated. The DNA methyltransferase inhibitor (5-Aza-CdR) effectively reversed the hypermethylation status of the promoter region. With demethylation, RKO cell growth was suppressed, the cells doubling times were prolonged (P<0.01) and apoptosis was induced, which showed a relationship. CONCLUSION A selective DNA methyltransferase (DNMT) inhibitor can inhibit proliferation by demethylation in 5'CpG islands, and may be a potential new therapy target for colorectal cancer.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.