The bacterial strain Lysinibacillus sp. (P-011) was isolated from the midgut of the Drosophila melanogaster larvae. The bacteria were gram positive, spore forming, rod shaped ranging from 1.86 to 2.5 μm in length and 0.50 to 0.67 μm in diameter, positive for catalase, indole, oxidase, nitrate reduction, starch and gelatin hydrolysis, sensitive to tetracycline, chloramphenicol, doxycycline hydrochloride, gatifloxacin, ofloxacin, vancomycin, rifampicin, levofloxacin, ciprofloxacin, nalidixic acid, but resistant to ampicillin, streptomycin, gentamycin and kanamycin. The phylogenetic tree showed that the strain Lysinibacillus sp. P-011 (GU288531) branched with Lysinibacillus boronitolerans with 89% bootstrap support. Lysinibacillus sp. P-011 (×10 5 cfu/ml) played an important role on larval development of D. melanogaster under controlled environmental condition. Wild larvae when fed on normal food as well as normal food mixed with ineffective antibiotics, developed puparium within seven days whereas took more than 10 days when fed on normal food mixed with anti P-011 antibiotics and sterile food mixed with bacterial suspension and anti P-011 antibiotics. 94 to 98% cured larvae developed puparium within seven days when fed on only sterile food mixed with bacterial suspension (P-011) or sterile food mixed with bacterial suspension (P-011) and ineffective antibiotics.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.