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The projects are funded under the 7 th framework programme and the LIFE + funding instrument of the European Commission. This poster does not constitute a formal communication and does not necessarily represent the official position of the European Commission or Member States. Nothing in this poster creates a binding provision. In case this poster is in conflict with the terms of the EC-GA, the terms of the latter shall prevail. Bisphenol A (BPA), triclosan (TCS) and 4-Nonylphenol (NP) are among endocrine disruptors which are widely used in daily products. BPA is mainly used to manufacture polycarbonate, while TCS is an antimicrobial and antifongical agent used in personal care, and n-NP is mainly present in detergent and cosmetic products. The aim of this work is to estimate the levels of BPA, TCS and NP in 131 urine samples collected from a non-occupationally exposed population aged from 1 to 75 years living in Liege (Belgium) and the surrounding areas, to estimate baseline values concentration in Belgium. Urinary I N T R O D U C T I O N 2. M A T E R I A L A N D M E T H O D POPULATION 65 men / 66 womenGrouped by age (± ± ± ± 11 subjects by class) BPA and TCS were positively detected in almost all samples (97.7% and 74.6% respectively).NP was not detected in any samples of urine analyzed, confirming that urinary NP (free or conjugated) may not adequately assess nonylphenol exposure.Both BPA and TCS levels were not correlated with creatinine excretion, questioning the relevance of the creatinine adjustment in reporting these chemical levels.BPA levels in urine of people living in the same home and collected on the same time were fairly correlated, confirming that dietary intake would be the primary route of exposure. TCS urinary levels were not correlated with BPA levels.No statistical difference was found neither for BPA and TCS levels between male and female. TCS concentrations were significantly higher for people aged between 20 and 39 years old.

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We report on the development and validation under ISO 17025 criteria of a multi-residue confirmatory method to identify and quantify 17 widely chemically different pesticides (insecticides: Carbofuran, Methiocarb, Pirimicarb, Dimethoate, Fipronil, Imidacloprid; herbicides: Amidosulfuron, Rimsulfuron, Atrazine, Simazine, Chloroturon, Linuron, Isoxaflutole, Metosulam; fungicides: Diethofencarb) and 2 metabolites (Methiocarb sulfoxide and 2-Hydroxytertbutylazine) in honey. This method is based on an on-column liquid-liquid extraction (OCLLE) using diatomaceous earth as inert solid support and liquid chromatography (LC) coupled to mass spectrometry (MS) operating in tandem mode (MS/MS). Method specificity is ensured by checking retention time and theoretical ratio between two transitions from a single precursor ion. Linearity is demonstrated all along the range of concentration that was investigated, from 0.1 to 20 ngg -1 raw honey, with correlation coefficients ranging from 0.921 to 0.999, depending on chemicals. Recovery rates obtained on home-made quality control samples are between 71 and 90%, well above the range defined by the EC/657/2002 document, but in the range we had fixed to ensure proper quantification, as levels found in real samples could not be corrected for recovery rates. Reproducibility is found to be between 8 and 27%. Calculated CCα and CCβ (0.0002-0.943 ngg -1 for CCα, and 0.0002-1.232 ngg -1 for CCβ) show the good sensitivity attained by this multi-residue analytical method. The robustness of the method has been tested in analyzing more than 100 raw honey samples collected from different areas in Belgium, as well as some wax and bee samples, with a slightly adapted procedure.

Parabens, benzophenone-3 (BP3), and phthalates are commonly used as antimicrobial conservator, UV-filter, and plasticizer, respectively, and are thought to exhibit endocrine disrupting properties. These endocrine disrupting activities have been recently assumed to lead to cutaneous malignant melanoma. Humans are exposed to these chemicals through different sources such as food, personal care products, or cosmetics. In this study, we measured urinary levels of 4 parabens, BP3, and 7 metabolites of phthalates in samples collected from 261 participants living in and around Liege (Belgium). The analyses were carried out by liquid chromatography tandem mass spectrometry (LC-MS/MS) using isotopic dilution. To the best of our knowledge, this is the first time that the urinary levels of these 3 classes of chemicals are reported for the same general population in Belgium. Most of the parabens, the BP3, and all the phthalate metabolites were detected in 82.8 to 100.0% of the samples. For most of these chemicals, the exposure patterns significantly differ not only between children and adults, but also between males and females, especially with higher concentrations of parabens and phthalate metabolites in female and children subjects, respectively.