No abstract

Visual control has an influence on postural stability. Whilst vestibular, somatosensoric and cerebellar changes have already been frequency analytically parameterized with posturography, sufficient data regarding the visual system are still missing. The aim of this study was to evaluate the influence of pathologic and simulated visual dysfunctions on the postural system by calculating the frequency analytic representation of the visual system throughout the frequency range F1 (0.03-0.1 Hz) of Fourier analysis. The study was divided into two parts. In the first part, visually handicapped subjects and subjects with normal vision were investigated with posturography regarding postural stability (stability effect, Fourier spectrum of postural sway, etc.) with open and closed eyes. The visually impaired and the normal group differed significantly in the frequency range F1 (p = 0.002). Significant differences of the postural stability between both groups were found only in the test position with open eyes (NO). The healthy group showed a significant loss of stability, whereas the impaired group showed an increased stability due to sufficient somatosensoric processes. Visually handicapped persons can compensate the visual information deficit through improved peripheral-vestibular and somatosensoric perception and cerebellar processing. In the second part, subjects with normal vision were examined under simulated visual conditions, e.g., hyperopia (3.0 D), reduced visual acuity (VA = 20/200), yoke prisms (4 cm/m) and pursuits (pendulum). Changes in postural parameters due to simulations have been compared to a standard situation (open eyes [NO], fixation distance 3 m). Visual simulations showed influence on frequency range F1. Compared to the standard situation, significant differences have been found in reduced visual acuity, pursuits and yoke prisms. A loss of stability was measured for simulated hyperopia, pendulum and yoke prisms base down. Stability regulation can be understood as a multi-sensoric process by the visual, vestibular, somatosensoric and cerebellar system. Reduced influence of a single subsystem is compensated by the other subsystems. Obviously the main part of reduced visual input is compensated by the vestibular system. Moreover, the body sway, represented by the stability indicator, increased in this situation.

The autonomic information flow (AIF) represents the complex communication within the Autonomic Nervous System (ANS). It can be assessed by the mutual information function (MIF) of heart rate fluctuations (HRF). The complexity of HRF is based on several interacting physiological mechanisms operating at different time scales. Therefore one prominent time scale for HRF complexity analysis is not given a priori. The MIF reflects the information flow at different time scales. This approach is defined and evaluated in the present paper. In order to aggregate relevant physiological time scales, the MIF of HRF obtained from eight adult Lewis rats during the awake state, under general anesthesia, with additional vagotomy, and additional betal-adrenergic blockade are investigated. Physiologically relevant measures of the MIF were assessed with regard to the discrimination of these states. A simulation study of a periodically excited pendulum is performed to clarify the influence of the time scale of MIF in comparison to the Kolmogorov Sinai entropy (KSE) of that well defined system. The general relevance of the presented AIF approach was confirmed by comparing mutual information, approximate entropy, and sample entropy at their respective time scales.

Reduced birth weight predisposes to cardiovascular diseases in later life. We examined in fetal sheep at 0.76 (n = 18) and 0.87 (n = 17) gestation whether spontaneously occurring variations in fetal weight affect maturation of autonomic control of cardiovascular function. Fetal weights at both gestational ages were grouped statistically in low (LW) and normal weights (NW) (P < 0.01). LW fetuses were within the normal weight span showing minor growth dysproportionality at 0.76 gestation favouring heart and brain, with a primary growth of carcass between 0.76 and 0.87 gestation (P < 0.05). While twins largely contributed to LW fetuses, weight differences between singletons and twins were absent at 0.76 and modest at 0.87 gestation, underscoring the fact that twins belong to normality in fetal sheep not constituting a major malnutritive condition. Mean fetal blood pressure (FBP) of all fetuses was negatively correlated to fetal weight at 0.76 but not 0.87 gestation (P < 0.05). At this age, FBP and baroreceptor reflex sensitivity were increased in LW fetuses (P < 0.05), suggesting increased sympathetic activity and immaturity of circulatory control. Development of vagal modulation of fetal heart rate depended on fetal weight (P < 0.01). These functional associations were largely independent of twin pregnancies. We conclude, low fetal weight within the normal weight span is accompanied by a different trajectory of development of sympathetic blood pressure and vagal heart rate control. This may contribute to the development of elevated blood pressure in later life. Examination of the underlying mechanisms and consequences may contribute to the understanding of programming of cardiovascular diseases.

We validated laser Doppler flowmetry (LDF) for long-term monitoring and detection of acute changes of local cerebral blood flow (lCBF) in chronically instrumented fetal sheep. Using LDF, we estimated developmental changes of cerebral autoregulation. Single fibre laser probes (0.4 mm in diameter) were implanted in and surface probes were placed on the parietal cerebral cortex at 105 ± 2 (n = 7) and 120 ± 2 days gestational age (dGA, n = 7). Basal lCBF was monitored over 5 days followed by a hypercapnic challenge (fetal arterial partial pressure of CO 2 , P a,CO 2 : 83 ± 3 mmHg) during which lCBF changes obtained by LDF were compared to those obtained with coloured microspheres (CMSs). Mean arterial blood pressure (MABP) was increased and decreased using phenylephrine and sodium nitroprusside at 110 ± 2 and 128 ± 2 dGA. Intracortical and cortical surface laser probes gave stable measurements over 5 days. The lCBF increase during hypercapnia obtained by LDF correlated well with flows obtained using CMS (r = 0.89, P < 0.01). The signals of intracortical and surface laser probes also correlated well (r = 0.91, P < 0.01). Gliosis of 0.35 ± 0.06 mm around the tip of intracortical probes did not affect the measurements. The range of MABP over which cerebral autoregulation was observed increased from 20-48 mmHg at 110 dGA to 35 to > 95 mmHg at 128 dGA (P < 0.05). Since MABP increased from 33 to 54 mmHg over this period (P < 0.01), the range between the lower limit of cerebral autoregulation and the MABP increased from 13 mmHg at 110 dGA to 19 mmHg at 128 dGA (P < 0.01). LDF is a reliable tool to assess dynamic changes in cerebral perfusion continuously in fetal sheep.