Berry consumption is associated with colorectal-cancer chemoprevention, but digestive conditions can affect this property. The bioaccessibility and apparent permeability coefficients of bioactive compounds from Andean Berry Juice (ABJ) after in vitro gastrointestinal digestion and colonic fermentation were analyzed. The antiproliferative effect of the fermented nondigestible fraction was evaluated against SW480 colon-adenocarcinoma cells. Gallic acid displayed the highest bioaccessibility in the mouth, stomach, small intestine, and colon. However, chlorogenic acid exhibited the highest apparent permeability coefficients (up to 1.98 × 10 cm/s). The colonic-fermentation fraction showed an increase of ≥50% antiproliferative activity against SW480 cells (19.32%, v/v), equivalent to those of gallic acid (13.04 μg/g), chlorogenic acid (7.07 μg/g), caffeic acid (0.40 μg/g), ellagic acid (7.32 μg/g), rutin (6.50 μg/g), raffinose (0.14 mg/g), stachyose (0.70 mg/g), and xylose (9.41 mg/g). Bioactive compounds from ABJ are bioaccessible through the gastrointestinal tract and colon fermentation, resulting in antiproliferative activity.
En primer lugar, a la Universidad Nacional de Colombia, por ser una magnifica institución de educación superior; permitiéndome formarme en áreas de la agricultura, el desarrollo rural y la docencia; a la Facultad de Ciencias Agrarias, mi segundo hogar, dónde me he formado profesionalmente; al CIER, por el apoyo económico para la fase de campo del presente trabajo.Al profesor Yesid Aranda por su dedicación y tiempo empleado en cada asesoría, de quién aprendí acerca de la constancia y la honestidad; así como, al profesor Gustavo Ligarreto, por su disponibilidad e interés en el trabajo. Asimismo, agradezco a la planta profesoral del Departamento de Desarrollo Rural, quienes mediante sus enseñanzas lograron orientarme en mi vida profesional.Un agradecimiento profundo, a los actores territoriales; donde destacan los campesinos, quienes compartieron sus saberes y experiencias de vida para el presente trabajo, así como, a los comercializadores y empresas transformadoras en el territorio.A las instituciones que hacen presencia en el territorio, donde destacan:
Vaccium meridionale Swartz, commonly Andean Berry, has a high content of several phytochemicals, such as anthocyanins, phenolic acids, and other flavonoids. However, in spite of its antioxidant capacity, there is little information about its anticarcinogenic properties. This study evaluated the antiproliferative and pro-apoptotic activity of Andean Berry Juice (ABJ) on human colon adenocarcinoma SW480 cells. The antiproliferative activity of ABJ was evaluated on SW480 cells using the Sulphorodamine B assay.The effect on cell viability, cytotoxicity and activation of caspase-3 was analyzed using The ApoTox-Glo™ Triplex Assay. Specific apoptotic biomarkers cleaved PARP, total Bcl-2-associated death promote (BAD), phosphorylated BAD, total p53, and phosphorylated p53 were also analyzed. To determine the intracellular redox-state, the Glutathione Assay Kit and 2′-7′-Dichlorodihydrofluorescein diacetate (DCFH-DA) were used, respectively. The antiproliferative assay showed a IC50 value of 8% v/v ABJ, the caspase 3 activity was increased in time-dependent manner in SW480 treated cells, the proapoptotic proteins (cleaved caspase 3, cleaved PARP, P53 and total BAD) were increased by 1.6 to 2.0 fold. In addition, the ABJ-treated SW480 cells increased significantly the production of intracellular reactive oxygen species (ROS), parallel with reduction in the intracellular content of glutathione (GSH) and consequently a decrease of GSH/ oxidized glutathione (GSSG) ratio. In conclusion, the ABJ was able to inhibit SW480 cells proliferation involving apoptotic mechanisms through the perturbation of intracellular oxidative state.
Objetivo: Comparar los efectos de dos planes de entrenamiento de Resistencia, con los métodos intermitente e interválico, durante 4 semanas con 4 estímulos por semana, en el VO2Máx en esgrimistas antioqueños, calculado de forma indirecta con el test de Legger. Método: un total de 9 deportistas (5 hombres y 4 mujeres) participaron en el presente estudio. La muestra fue dividida en 2 grupos experimentales (G1 y G2) y 1 grupo Control. El plan de entrenamiento consistió en una intervención de carácter aeróbica equivalente a 20 minutos, ambos grupos realizaron 4 intervenciones por semana durante 4 semanas. Resultados: no se observan diferencias estadísticamente significativas entre quienes realizaron método intermitente o interválico pero se observa una tendencia a mejorar en Intermitente y se mantienen todos los valores de los atletas evaluados en el interválico. Conclusiones: En el desarrollo de bloques concentrados para entrenar la resistencia en el período competitivo es adecuado intervenir a los deportistas con los métodos: intermitente o interválico, ya que mantendrán o mejorarán el VO2Máx.
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