The Kiss1/GPR54 system has recently been shown to play a key role in the onset of puberty in mammals. Growing evidence suggests that this system is also conserved across vertebrates although very few studies so far have been performed in lower vertebrates. The aims of this study were firstly in the teleost Nile tilapia to screen tissues for GPR54 expression levels, secondly to measure the expression patterns of GPR54 and GnRH I receptor (rGnRH I) in whole brains during the onset of puberty and finally to determine the effects of continuous illumination (LL) on receptor expression levels. Results confirmed that GPR54 was predominantly expressed in the brain and pituitary of adult tilapia. Furthermore, a significant increase of GPR54 gene expression was found in tilapia brains at 11 weeks post hatch (wph) followed by rGnRH I at 13 wph just prior to the histological observation of vitellogenic oocytes and active spermatogenesis in ova and testes at 17 wph. These results suggest a correlation between the increase of GPR54 expression in the brain and the onset of puberty. Finally, a significant effect of LL was observed on GPR54 expression levels which were characterized by a delayed surge with significantly lower levels than those of control fish. The current study not only suggests a link between the Kiss1/GPR54 system and the onset of puberty in a tropical batch spawning teleost that would be a highly conserved feature across vertebrates but also that the transcriptional mechanisms regulating GPR54 expression could be directly or indirectly influenced by light

The purpose of this work was to investigate the circadian melatonin system in two tropical teleost species characterised by different behavioural habits, Nile tilapia (diurnal) and African catfish (nocturnal). To do so, fish were subjected to either a control photoperiod (12L:12D), continuous light (LL) or darkness (DD) or a 6L:6D photoperiod. Under 12L:12D, plasma melatonin levels were typically low during the photophase and high during the scotophase in both species. Interestingly, in both species melatonin levels significantly decreased prior to the onset of light, which in catfish, reached similar basal levels to those during the day, demonstrating that melatonin production can anticipate photic changes probably through circadian clocks. Further evidence for the existence of such pacemaker activity was obtained when fish were exposed to DD, as a strong circadian melatonin rhythm was maintained. Such an endogenous rhythm was sustained for at least 18 days in Nile tilapia. A similar rhythm was shown in catfish although DD was only tested for four days. Under LL, the results confirmed the inhibitory effect of light on melatonin synthesis already reported in other species. Finally, when acclimatised to a short photo-cycle (6L:6D), no endogenous melatonin rhythm was observed in tilapia under DD, with melatonin levels remaining high. This could suggest that the circadian clocks cannot entrain to such a short photo-cycle. Additional research is clearly needed to further characterise the circadian axis in teleost species, identify and localize the circadian clocks and better understand the environmental entrainment of fish physiology.3

The aim of this work was to confirm previous findings suggesting that the eyes are required for night-time melatonin production in Nile tilapia and further characterise this divergent circadian organisation. To do so, melatonin levels were firstly measured in eyecups and plasma to determine circadian patterns of melatonin production.Secondly, the effect of partial ophthalmectomy on the suppression of melatonin production was determined in vivo as well as ex vivo pineal light/dark sensitivity.Finally, to investigate whether such findings could be related to post-surgery stress, melatonin analyses were performed in the subsequent 24 h and 7 days postophthalmectomy with cortisol levels assessed as an indicator of stress. Our results showed an inverse pattern of melatonin production in the eye cups of tilapia compared to blood circulating levels, suggesting different roles played by melatonin in these two tissues. Results then demonstrated that total or partial ophthalmectomy resulted in the suppression of night-time melatonin production. Furthermore, although pineals in culture were shown to be photosensitive, night-time melatonin levels were much lower than seen in other species. Finally, when performing sampling immediately or one week post-surgery, no difference in the melatonin profiles were observed. It is therefore unlikely that post-surgery stress would explain such suppression in melatonin production although all fish displayed high cortisol levels most probably due to social and handling stress. Taken together, these results provide further evidence of a new type of circadian organisation in a teleost species where the eyes are required to sustain night time melatonin levels. Section: Regulatory systems