SummaryHeterozygous loss-of-function mutations in GRIN2B, a subunit of the NMDA receptor, cause intellectual disability and language impairment. We developed clonal models of GRIN2B deletion and loss-of-function mutations in a region coding for the glutamate binding domain in human cells and generated neurons from a patient harboring a missense mutation in the same domain. Transcriptome analysis revealed extensive increases in genes associated with cell proliferation and decreases in genes associated with neuron differentiation, a result supported by extensive protein analyses. Using electrophysiology and calcium imaging, we demonstrate that NMDA receptors are present on neural progenitor cells and that human mutations in GRIN2B can impair calcium influx and membrane depolarization even in a presumed undifferentiated cell state, highlighting an important role for non-synaptic NMDA receptors. It may be this function, in part, which underlies the neurological disease observed in patients with GRIN2B mutations.
To date, information systems (IS) research mainly has provided a monolithic view of information technology (IT) use, considering it to be a desired behaviour with positive outcomes. However, given the dramatic increase in the use of technology during the last few years, susceptibility to IT addiction is increasingly becoming an important issue for technology users and IS researchers. In this paper, we report the results of a study that focuses on identifying variations in user liability to IT addiction, which reflects the susceptibility of individual users to develop IT addiction. First, a review of the literature in different disciplines (e.g. health, psychology and IS) allows us to better understand the concepts of IT addiction and liability to addiction. The literature review also provides an overview of the antecedents and consequences associated with IT addiction. Then, building on the analysis of 15 in-depth interviews and 182 exploratory open-ended surveys collected from smartphone users, we apply the concept of liability to addiction in the IT use context and propose a typological theory of user liability to IT addiction. Our typology reveals five ideal types; each can be associated to a user profile (ADDICT, FANATIC, HIGHLY ENGAGED, REGULAR AND THOUGHTFUL). Building upon both the extant literature and our results, we put forth propositions to extend the theoretical contributions of the study. We finally discuss the contributions and implications of our paper for research and practice.
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