Contents The aim of the present study was to investigate whether or not the size of the ovarian fragment influences its resistance to cryostorage. For that purpose, ovaries were collected from 34 queens (various breeds, age 1–5 year) by routine ovariectomy, transported to the laboratory and then sectioned in different sizes (3 mm × 3 mm × 3 mm, 5 mm × 3 mm × 3 mm and 7 mm × 3 mm × 3 mm) and randomly assigned to a control (GC3, GC5 and GC7, respectively) or vitrified (GV3, GV5 and GV7, respectively) groups. Vitrified‐warmed fragments were evaluated by histomorphology and immunohistochemistry (for apoptotic rates by using cleaved caspase‐3). Histological examination reveals that 72.97% of the follicles in GV3 and 72.58% in GV5 were normal while only 42.86% of the follicles in GV7. The main morphological alteration presented in all groups was a detachment of the epithelial cells. Similarly, immunohistochemistry evaluation using caspase 3 revealed a small proportion of apoptotic cells in GV3 (8.43%) while in GV7 30.43% of the cells expressed cleaved caspase‐3. These findings indicate that fragments sectioned in 3 mm × 3 mm × 3 mm (27 mm3) seem more adequate for perfusion of the cryoprotectant, causing less damage to the cell after vitrification‐warming.
RESUMO:A ultrassonografia possui grande importância na clínica veterinária de pequenos animais. Além de auxiliar no diagnóstico de afecções, apresenta ampla valia no diagnóstico gestacional de cadelas. Por ser um método precoce, não invasivo, indolor e sem riscos para a gestante e os neonatos, favorece o acompanhamento destes, bem como avalia a viabilidade fetal e detecta possíveis anormalidades. O presente estudo objetivou analisar a eficácia da ultrassonografia durante a gestação da cadela; utilizar parâmetros para determinar a idade gestacional e estimar a data do parto. Foram utilizadas 29 cadelas gestantes de raças variadas para acompanhamento ultrassonográfico em dias aleatórios, conforme rotina hospitalar, sendo as medidas fetométricas registradas e os parâmetros, mais frequentes, utilizados para estimativa da provável data de parto. De acordo com os dados obtidos, pode-se concluir que o ultrassom é método eficaz no diagnóstico da gestação, porém requer treinamento de técnica e habilidade. Já a biometria fetal permite estimar a idade do concepto, através da aplicação da equação de regressão linear obtida no presente trabalho.Palavras-chave: Fetometria. Prenhez. Cães. OBSTETRICAL ULTRASOUND IN BITCHESSUMMARY: Ultrasonography shows a lot of importance in veterinary clinical of small animals. Besides helping in the diagnosis of pathologies, shows great importance in gestational diagnosis of bitches. By being early method, non-invasive, painless and without risk to pregnant and neonates, favors the monitoring of these, assessment of fetal viability and detection of possible abnormalities. The present study aimed to analyze the effectiveness of ultrasound during canine female pregnancy; to use parameters for determine gestational age and predict the likely delivery date. Therefore 29 pregnant dogs of varied breeds were used to ultrasound scaning on random days, according to hospital routine. Fetal biometry measurements were recorded and the most frequent parameters were used to estimate the date of birth. According to results obtained, can be concluded that ultrasound is an effective method in the diagnosis of gestation, but it requires technical training and ability. However, fetal biometry allows estimate the age of the concept, through the application of the linear regression equation obtained in the present work. Keywords
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