Although coevolution is widely accepted as a concept, its importance as a driving factor in biological diversification

Pollinator-mediated selection has been suggested as a key driver of speciation in plants. We examined the potential role of hawkmoth pollinators in driving allopatric divergence and maintaining sympatric coexistence of morphotypes in the African iris Pollinator-mediated selection plays a key role in shaping flower morphology

Although the tremendous variability in floral colour among angiosperms is often attributed to divergent selection by pollinators, it is usually difficult to preclude the possibility that floral colour shifts were driven by non-pollinator processes. Here, we examine the adaptive significance of flower colour in Disa ferruginea, a non-rewarding orchid that is thought to attract its butterfly pollinator by mimicking the flowers of sympatric nectar-producing species. Disa ferruginea has red flowers in the western part of its range and orange flowers in the eastern part-a colour shift that we hypothesized to be the outcome of selection for resemblance to different local nectar-producing plants. Using reciprocal translocations of red and orange phenotypes as well as arrays of artificial flowers, we found that the butterfly Aeropetes tulbaghia, the only pollinator of the orchid, preferred both the red phenotype and red artificial flowers in the west where its main nectar plant also has red flowers, and both the orange phenotype and orange artificial flowers in the east, where its main nectar plant has orange flowers. This phenotype by environment interaction demonstrates that the flower colour shift in D. ferruginea is adaptive and driven by local colour preference in its pollinator.

Summary• Plant adaptations to pollinators are usually studied at the species level, but are expected to occur at the local population level and be reflected in fine-scale patterns of floral variation.• Here, we examined whether a guild of c. 20 South African plant species pollinated by the long proboscid fly Prosoeca ganglbaueri (Nemestrinidae) exhibits fine-scale patterns of geographical covariation and convergent evolution at a local scale.• Fly proboscis length is highly variable among sites (20-50 mm). Plant adaptation results in floral depths of plants within the guild being closely matched with the proboscis length of their fly pollinator across numerous sites. This results in patterns of divergence among allopatric populations and convergence among species within a site.• The most likely evolutionary processes driving these patterns include coevolution between the fly and plants with consistent and abundant rewards, as well as onesided evolution in rare and nonrewarding species that do not influence the coevolutionary process. Pollinator-mediated selection on spur length was confirmed for a nonrewarding orchid species in the guild by a reciprocal translocation experiment. Thus, rarer and nonrewarding species in the guild are forced to keep pace with the coevolutionary race between common rewarding flowers and flies.