ResumoA imagem da capa é um dos produtos da oficina fotográfica realizada durante o projeto integrado de ensino, pesquisa e extensão “Expressões Potentes na Escola Pública: corpo e arte”, do Laboratório de Atividades Humanas e Terapia Ocupacional. Esta atividade foi idealizada e produzida pela coordenadora e os estudantes de graduação e pós-graduação que atuavam na equipe em parceria com os estudantes de uma escola pública na cidade de São Carlos-SP, em 2017. A Oficina de Atividades teve como objetivo trabalhar com as questões sobre o pertencimento e a autoimagem dos participantes, dar expressão às suas essências. O processo de produção das imagens, em associação com as demais estratégias construídas durante o projeto inclusive as relações de afeto, demonstraram ser potentes e sensíveis para o protagonismo dos envolvidos através de um espaço apropriado para a expressão de ideias, sentimentos e emoções, que promovesse o respeito de si e do outro. Abstract The cover image is one of the photographic workshop products produced during the integrated teaching, research and extension project "Powerful Expressions in Public School: Body and Art", from the Human Activities and Occupational Therapy Laboratory. This activity was conceived and produced by the coordinator, undergraduate and graduate students who worked in the team in partnership with the students of a public school in the city of São Carlos-SP, in 2017. The Activities Workshop had as objective to work with questions about the participants' self-image and belonging, and offer spaces for the expression of their essences. The image production process, in association with the other strategies built during the project, including affective relations, proved to be potent and sensitive for the protagonism of those involved through an appropriate space for the expression of ideas, feelings and emotions, to promote the respect of self and the other.Keywords: Body; Culture; Photography; Respect; Occupational Therapy.
Introduction:The SARS-CoV-2 pandemic has spurred the textile industry to develop functional textiles formulated with antiviral agents, first conceived to produce personal protective equipment. The textiles antiviral efficacy need to be evaluated and approved. Therefore, to assure the results it is essential to validate viral quantification methodologies based on ISO 18184. Furthermore, this validated TCID 50 is offered by Bio-Manguinhos to SENAI CETIQT partner as a technological service to measure antiviral activity of fabrics produced by national companies.Objective: This study aims to assess and to validate the TCID 50 as a toll to measure the antiviral efficacy of functional textiles.Methodology: Analytical performance was established under cell-based assay characteristics using a model virus. Briefly, the Measles Schwarz stock solution was diluted and quantified by TCID 50 . Assays were performed by different operators and days to assess linearity, accuracy, and precision. Textile samples were also evaluated for cytotoxicity and antiviral activity to determine selectivity and robustness. Statistics were performed with the software R Studio. Results:The theoretical viral titer was calculated and compared with experimentally TCID 50 titer obtained and linear regression analysis showed a significant correlation between both (p < 0,001; R2= 0.98; R= 0.99) with high accuracy. Neither the treated nor control fabrics induced cytotoxicity or loss of cellular sensibility to infection, according to the criteria down to 0.5 Log TCID 50 /mL. The method to quantify antiviral activity was robust with minimal variation in all samples inoculated with different volume inoculum and washing medium. Conclusion:Our findings represent an important step regarding the development of methods able to infer virus titers and antiviral activity in the field of fabrics. The validated TCID 50 to quantify antiviral activity demonstrated precision, accuracy and robustness, has supported the SENAI CETIQT in the development and validation of the effectiveness of functional textiles used by society and its implementation has represented the first technological service in Bio-Manguinhos.
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