Intestinal folacin absorption and mucosal conjugase activities were assessed in healthy male elderly (65-83 years) and young (20-29 years) human subjects. Luminal disappearance as measured simultaneously from the perfused jejunum of 3 mumol of both [3H]pteroylmonoglutamate [( 3H]PG-1) and pteroyl-[14C]glutamyl-hexaglutamate [( 14C]PG-7) as well as the urinary recovery of each isotope and mucosal conjugase activities were compared. Luminal disappearance of either [3H]PG-1 or [14C]PG-7 was not different for the two age groups (P greater than 0.05). The average (mean +/- SD) disappearance of [3H]PG-1 was 63 +/- 12% in the elderly and 67 +/- 6% in the young groups. In contrast, the disappearance of [14C]PG-7 averaged 51 +/- 6% in the elderly and 48 +/- 3% in the young group. Urinary recovery (percent in 48 hours) was not different for either isotope when the two age groups were compared (P greater than 0.05). The urinary recovery of 3H averaged 60 +/- 21% in the elderly and 59 +/- 13% in the young in contrast to 35 +/- 22% and 35 +/- 7% for 14C recovery in the elderly and young groups, respectively. Folacin conjugase activities measured at pH 6.5 and at pH 4.5 were not different between the groups (P greater than 0.05). The findings indicate that intestinal absorption of folacin and activity of folacin conjugases are not affected by the aging process.
Mastitis due to Protothecazopfii was diagnosed in three of 28 cows in a dairy herd. As two cows continued to shed algae after 45 days they were slaughtered and organs were examined by cultivation, histology, immunohistochemistry and electron microscopy. Algae were restricted to the mammary glands and regional lymph nodes in which a granulomatous inflammation was seen. Algae were predominantly seen in macrophages but neutrophils also contained organisms. In macrophages both sporangiospores and sporangia were found, suggesting that intracellular proliferation may be responsible for the failure to overcome the infection. Serum samples from all cows were assayed for antibodies against P.zopfii in an enzyme‐linked immunosorbent assay (ELISA). Although the highest titre was found in an infected cow the difference between the mean values of the titre in infected and non‐infected cows was not significant.
Wenn man von melanotischen, yon der Epidermis ausgehenden Tumoren spricht, so toeing man damit wohl fast stets, und dem in der Literatur vorliegenden Material naeh auch mit Recht, Neubildungen, welehe sich aus Naevuszellen oder ihren malignen Derivaten zusammensetzen oder sigh wenigstens --wit die Hutchinson-Dubreuilhsehen Melanome (Lentigo malin des vieillards) --im Charakter der Zellen und Aufbau des Tumors den Naevocarcinomen eng anschliel3en.Die folgende Mitteilung betrifft einige zufgllig von mir beobaehtete FMIe, bei welehen, klinisch sowohl wie histologiseh, das melanotisehe Moment ebenso stark im Vordergrund steht, wit beim Pigmentnaevus und den yon ihm ausgehenden Neubildungen, so dal~, wenigstens kliniseh, diese Diagnose am niichsten liegt. Die Struktur und der Zelleharakter unterseheiden aber diese F~lle sowohl voneinander als yore eigentlichen Naevus, indem die Iiir letztern eharakteristisehen histologisehen Vorg~nge und Naevuszellen vollkommen fehlen. Da mir gleiehe Fglle aus der Literatur night bekannt sind und die Befunde Anlag zur ErSrterung einiger noeh strittiger Fragen aus der Pigmentlehre bitten, so halte ieh mich flit bereehtigt, sit bier etwas eingehender zu besehrieben.
Fall.Klinisches. Die Affektion bes~eh} naeh den Angaben des 74j~hrigen Pat, seit ca. 12 Jahren und hat sieh w~hrend dieser Zeit aus einer urspriinglich etwa linsengrol?en solitgren Papel zu der jetzigen GrSge entwickelt. Als ieh den Pat. zum erstenmal sah, handelte es sich um einen etwa halbhandtellergroBen Herd auf der linken lateralen Thoraxseite. Dieser Herd best~nd aus ca. 8 erbsen-bis bohnengroBen, flaeh erhabenen, das Niveau der Haut 4--5 mm iiberragenden Papeln, mit deutlieh eircingrer Anordnung. Im Zentrum des ganzen Herdes war die Haul etwas eingesunken, braun rStlieh, Iein atrophiseh. Datum herum, vor-
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