A field experiment was conducted under irrigated conditions in the highlands of Madagascar to assess the potential of intercropping Italian ryegrass with common vetch for improving yield and quality of forage. Seed proportions studied were ryegrass-vetch 100:0; 0:100; 50:33; 50:50; 50:66; 75:33 and 75:66. Mixtures were sown in alternate rows and the sowing rates of pure stands of ryegrass and vetch were 20 and 60 kg per hectare, respectively. The results showed that all mixtures achieved yield advantage over pure stands with the highest land equivalent ratio value for dry matter yield obtained from the mixture of 75:66 (1.47) followed by 50:50 (1.35). Slight increase of crude protein content and protein digested in the small intestine when rumen-fermentable nitrogen is limiting (PDIN) content were obtained from mixtures compared with pure stand of ryegrass. Vetch rate in dry matter yield of mixtures increased with the increase of vetch seed proportion and ranged from 31 to 44%. Agressivity and competitive ratio indices showed that ryegrass was slightly competitive than vetch. Intercropping Italian ryegrass with common vetch at the seed proportions of 75:66 or 50:50 could be a more sustainable alternative cropping to alleviate dry season feed shortages of dairy livestock in the highlands of Madagascar.
Population growth and urban development in the South renew research topics on agriculture. Advantageous in some respects, urban agriculture entails many risks, especially those related to the quality of irrigation water and to urban farmers’practices. The article illustrates the case of Antananarivo, the capital of Madagascar, where watercress, which is highly appreciated by the consumers, had a growing development in the lowland of the city. This activity creates high income and can be practiced all around the year. However, health risks associated with its conditions of production are numerous : use of waste water and urban effluents, questionable practices of some farmers and traders. A multidisciplinary research program has studied this situation. A survey on consumption showed that methods used by households from Antananarivo for watercress preparation are suitable. Furthermore, microbiological analyses of the product at the production level and in households are reassuring. However, some issues remain unresolved. A qualitative survey reveals a duality in the image of the product : consumers are indeed shared between, firstly, their good appreciation of organoleptic and nutritional qualities of watercress and, secondly, their fear of disease related to the conditions of production they witness. The problem of safety is real because of the subjectivity of consumers that could, at worst, turn away from this product, at best, ask questions about the quality of their diet.La croissance démographique et le développement urbain des pays du Sud renouvellent les thématiques de recherche sur l’agriculture. Avantageuse par certains côtés, l’agriculture urbaine présente plusieurs risques, notamment liés à la qualité des eaux d’irrigation qu’elle utilise et aux pratiques des agriculteurs urbains. L’article témoigne du cas d’Antananarivo, capitale de Madagascar, où l’activité cressonnière s’est particulièrement développée dans les bas fonds de la ville et dont les habitants consomment des quantités importantes de cresson. Cette activité est créatrice de revenus conséquents et peut être pratiquée toute l’année. Cependant, les risques sanitaires associés aux conditions de production sont nombreux : eaux d’irrigation réceptacles d’effluents urbains, pratiques polluantes ou peu hygiéniques de certains cressiculteurs et commerçants, etc. Un programme de recherche multidisciplinaire étudie cette situation. Une enquête de consommation a montré que les modalités de préparation du cresson par les ménages antananariviens semblent adaptées aux caractéristiques du produit disponible et les analyses microbiologiques du cresson sur quelques sites sont rassurantes. Cependant, certaines questions restent encore en suspens. Une enquête qualitative révèle qu’il existe une dualité en matière d’image du produit : les consommateurs sont partagés entre, d’une part, leur bonne appréciation des qualités organoleptiques et nutritionnelles du cresson et, d’autre part, leur crainte de maladie au regard des conditions de production dont ils sont té...
Pseudomonas aeruginosa has the ability to resist almost all available antibiotics by rapidly accumulating multiple resistance mechanisms and thus lead to a therapeutic impasse and higher mortality in infected patients. The objective of this study was to assess the phenotypic variation in resistance to tobramycin and ofloxacin from Pseudomonas aeruginosa by repeated exhibition after determination of the minimum inhibitory concentration. This is a prospective and descriptive study carried out in the Laboratory of Microbiology of Fundamental and Applied Biochemistry (Faculty of Sciences, Antananarivo) during the month of January 2020. The strains studied were the virulent wild strain of Pseudomonas aeruginosa PAO1 supplied by the Laboratory and two clinical strains of Pseudomonas aeruginosa from the Microbiology Laboratory of the Joseph Ravoahangy Andrianavalona University Hospital Center, Antananarivo. The strains of P. aeruginosa were cultured in the liquid culture medium (which is Luria Bertani, added with a buffer system of 3- (N-morpholino) propanesulfonic acid (LB-MOPS) which will stabilize the pH and a solid culture medium which is Columbia agar. Repeated exhibition to Tobramycin and Ofloxacin from these strains have been made. The MIC is determined by a visual evaluation of the turbidity of the various wells of the microplate. The MIC value of Pseudomonas aeruginosa with tobramycin and ofloxacin is very variable for the initial MIC until the 5th generation after repeated exhibition. More Pseudomonas aeruginosa is exposed to an antibiotic many times, the more it develops resistance to this antibiotic, even being sensitive at the start. That is to say, clinically, the dose prescribed for the antibiotic has been greatly exceeded if Pseudomonas aeruginosa is repeatedly exposed to the same antibiotic.
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