In this work, iminodiacetic acid (IDA) functionalized magnetic nanoparticles (Fe3O4@SiO2@IDA) were prepared and the adsorption behavior of Cr(iii)/(vi) on them was investigated.
A ratiometric DNA
sensor was developed based on fluorescent silicon
nanodots (SiNDs) and Ru(bpy)2(dppx)2+. The absorption
spectrum of Ru(bpy)2(dppx)2+ has significant
overlap with both the excitation and emission spectra of SiNDs. Therefore,
fluorescence quenching of Ru(bpy)2(dppx)2+ toward
SiNDs can occur on account of the strong inner filter effect. The
effect of quenching is not influenced by the specific binding between
Ru(bpy)2(dppx)2+ and DNA. Fluorescence turn-on
detection of DNA can be performed employing Ru(bpy)2(dppx)2+ and SiNDs as the response and reference signals, respectively.
Using SiND–Ru(bpy)2(dppx)2+, a convenient,
sensitive, rapid, and precise method could be developed for DNA detection.
In aqueous solutions, the I
601/I
448 fluorescence intensity ratio of SiND–Ru(bpy)2(dppx)2+ increases linearly in the DNA concentration
range of 20–1500 nM. The limit of detection and precision of
the method is 4.3 nM and 3.5% (50 nM, n = 13), respectively.
The ratiometric sensor was tested for visual detection of trace DNA.
Moreover, this method was found suitable for the ratiometric detection
of DNA in a simulated sample and a human serum sample, and the recoveries
were in the range of 98–119%.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.