A piezoelectric immunosensor was developed for the detection of SARS-associated coronavirus (SARS-CoV) in sputum in the gas phase. Horse polyclonal antibody against SARS-CoV was bound onto the PZ crystal surface in an ordered orientation through protein A. The antigen sample was atomized into aerosol by an ultrasonator, by which the antibody on the crystal could specifically adsorb SARS antigen and the changed mass of crystal would lead a frequency shift. A frequency counter was employed to record the admittance frequency, and the plot of changed frequency was displayed on the computer. Under the optimized conditions, the frequency shifts were linearly dependent on antigen concentration in the range of 0.6-4 microg/mL. The device has good reproducibility (could be reused 100 times without detectable loss of activity), stability (the immunosensor was stable for more than two months when stored over silica gel blue at 4-6 degrees C), short analyzing time (less than 2 min), and specificity.
This work discusses the preparation of small metal nanoclusters stabilized with glycol and OH )1 , which are attractable building blocks for the fabrication of new heterogeneous catalyst. New Os and Pt/Rh bimetallic nanoclusters with small particle size and narrow size distribution have been prepared by the polyol synthesis method and characterized by TEM, EDX, XRD and XPS. Superior catalytic properties for the selective hydrogenation of chloronitrobenzene over the nanocomposite catalysts composed of metal nanocluters and inorganic semiconductor nanoparticles have been discussed.
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