A new steroidal saponin was isolated from the leaves of Agave attenuata Salm-Dyck. Its structure was established as (3 ,5 ,22α,25S)-26-( -d-glucopyranosyloxy)-22-methoxyfuros-The structural identification was performed using detailed analyses of 1 H and 13 C NMR spectra including 2D NMR spectroscopic techniques (COSY, HETCOR and COLOC) and chemical conversions. The haemolytic potential of the steroidal saponin was evaluated and the anti-inflammatory activity was performed using the capillary permeability assay.

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