A novel low power, low cost, highly sensitive, miniaturized light emitting diode (LED) based flow detector has been used as optical detector for the detection of sample components in high performance liquid chromatography (HPLC). This colorimetric detector employs two LEDs, one operating in normal mode as a light source and the other is reverse biased to work as a light detector. Instead of measuring the photocurrent directly, a simple timer circuit is used to measure the time taken for the photocurrent generated by the emitter LED (λ max 500 nm) to discharge the detector LED (λ max 621 nm) from 5 V (logic 1) to 1.7 V (logic 0) to give digital output directly without using an A/D converter.Employing a post-column reagent method, a Nucleosil 100-7 (functionalised with iminodiacetic acid (IDA) groups) column was used to separate a mixture of transition metal complexes, manganese (II) and cobalt (II) in PAR. All optical measurements were taken by using both the in built HPLC variable wavelength detector and the proposed paired-emitter- * Correspondence author: Tel.: +353-1-7005404; fax: +353-1-7008002 email: dermot.diamond@dcu.ie
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