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Background Previous studies report that lipopolysaccharide (LPS)-preconditioned mesenchymal stem cells have enhanced trophic support and improved regenerative and repair properties. Extracellular vesicles secreted by synovial mesenchymal stem cells (EVs) can reduce cartilage damage caused by osteoarthritis (OA). Previous studies show that extracellular vesicles secreted by LPS-preconditioned synovial mesenchymal stem cells (LPS-pre EVs) can improve the response to treatment of osteoarthritis (OA). This study sought to explore effects of LPS-pre EVs on chondrocyte proliferation, migration, and chondrocyte apoptosis, as well as the protective effect of LPS-pre EVs on mouse articular cartilage. Methods Chondrocytes were extracted to explore the effect of LPS-pre EVs on proliferation, migration, and apoptosis of chondrocytes. In addition, the effect of LPS-pre EVs on expression level of important proteins of chondrocytes was explored suing in vitro experiments. Further, intraarticular injection of LPS-pre EVs was performed on the destabilization of the medial meniscus (DMM)-induced mouse models of OA to explore the therapeutic effect of LPS-pre EVs on osteoarthritis in vivo. Results Analysis showed that LPS-pre EVs significantly promoted proliferation and migration of chondrocytes and inhibited the apoptosis of chondrocytes compared with PBS and EVs. Moreover, LPS-pre EVs inhibited decrease of aggrecan and COL2A1 and increase of ADAMTS5 caused by IL-1β through let-7b. Furthermore, LPS-pre EVs significantly prevented development of OA in DMM-induced mouse models of OA. Conclusions LPS pretreatment is an effective and promising method to improve therapeutic effect of extracellular vesicles secreted from SMSCs on OA.

An elastic cloak is a coating material that can be applied to an arbitrary inclusion to make it indistinguishable from the background medium. Cloaking against elastic disturbances, in particular, has been demonstrated using several designs and gauges. None, however, tolerate the coexistence of normal and shear stresses due to a shortage of physical realization of transformation-invariant elastic materials. Here, we overcome this limitation to design and fabricate a new class of polar materials with a distribution of body torque that exhibits asymmetric stresses. A static cloak for full two-dimensional elasticity is thus constructed based on the transformation method. The proposed cloak is made of a functionally graded multilayered lattice embedded in an isotropic continuum background. While one layer is tailored to produce a target elastic behavior, the other layers impose a set of kinematic constraints equivalent to a distribution of body torque that breaks the stress symmetry. Experimental testing under static compressive and shear loads demonstrates encouraging cloaking performance in good agreement with our theoretical prediction. The work sets a precedent in the field of transformation elasticity and should find applications in mechanical stress shielding and stealth technologies.

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