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In 12 children aged four-and-a-half to 18 years (mean 11 ±4.2) undergoing continuous ambulatory peritoneal dialysis (CAPO), serum intact parathyroid hormone (iPTH), ionized calcium (iCa) levels, and calcium mass transfer (CaMT) were measured on three consecutive days: day 1, after a four-hour interval between dialyses; on day 2, after four hours dwell time with peritoneal dialysis (PD) Ca 3.5 mEq/L; and on day 3, after four hours dwell time with PD Ca 2.5 mEq/L. A significantly more negative CaMT was found when PD Ca 2.5 mEq/L was used, as compared with values obtained using PD Ca 3.5 mEq/L. Significantly lower parathyroid hormone (PTH) values were found after the interval between exchanges. We conclude that in order to properly evaluate parathyroid gland function and to decide whether or not to give vitamin D metabolites, a protocol for determining PTH should be standardized.
Hesperidin (HE), chrysin (CH) and naringenin (NA) are flavonoids, being the most important group of polyphenols, and show anti-inflammatory properties which have been demonstrated on various models. Polyphenols have a lot of biological properties, such as antioxidative, antiviral, immunomodulatory and anticancer activities. However, the effect on mastitis has not been yet described. This research aimed to analyze the tolerability of selected polyphenols after multiple intramammary administrations (IMM) as well as to investigate their potential impact on somatic cell count (SCC) in mastitis dairy cows. The study was performed on 12 Polish Holstein Black-White cows in their 4th – to 6th lactation. Only animals with inflammation in one-quarter of the udder were selected. The selection was based on SCC and clinical assessment. The experiment was performed with multiple IMM administrations with each of these polyphenols in dairy cows affected with mastitis. Polyphenols were administered at a dose of 30 mg/quarter/day. Milk samples for SCC, blood plasma samples for pharmacokinetics and blood hematology and biochemistry (selected blood parameters were tested) were collected at baseline, treatment period and within the recovery period. Positive effects concerning the number of SCC in milk of mastitic cows were confirmed for all tested polyphenols.
Baicalin is a flavonoid that has an influence on molecular processes. It possesses anticancer, anti-inflammatory, antiviral, antioxidative, and antithrombotic properties. It was found that baicalein treatment attenuated the damage of the mammary gland induced by LPS, suppressed the activity of myeloperoxidase, TNFα, and IL-1β in mice with mastitis. The aim of the study was a pilot analysis of baicalin tolerability after intramammary (IMM) administration and its impact on somatic cell count (SCC) after multiple IMM treatment on dairy cows with clinical mastitis. Moreover, the determination of baicalin in milk was performed by the sensitive ultra-high performance liquid chromatography with tandem mass spectrometry. The pharmacokinetic analyses were performed using Phoenix® WinNonlin® 6.4 and ThothPro v 4.1 software. Twelve dairy cows with clinical mastitis were selected for this study. The pharmacodynamic endpoint was SCC level and the clinical investigation was also carried out. Baseline SCC analysis was performed every 24 h among all cows three days before the first dose (B1–B3). After the baseline monitoring, 8 days of treatment (T1–T8) was performed and 8 days within recovery period SCC level was observed (R1–R8). Starting from T1 to T8, a decrease of SCC in relation to baseline was characterized by a declining trend. The presented results confirm the effect of baicalin on the reduction of SCC in mastitis in dairy cows after this therapy. The current study has shown that baicalin accumulation was not confirmed.
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