A simple, rapid, accurate and precise spectrophotometric method is proposed for the determination of thiamine hydrochloride (Vitamin B 1) in both pure form and in its pharmaceutical formulations. The method is based on the oxidation-reduction reaction between vitamin B 1 and known amount of chromate CrO 4-2 in acidic medium of 2N H 2 SO 4. Then, the excess of chromate is measured via 1,5-diphenylcarbazide which gives a pinkish-violet, water soluble and stable complex and exhibits maximum absorption at 543 nm, with a molar absorptivity of 1.5×10 4 l. mol-1. cm-1 , Sandell's sensitivity index of 0.02248 µg.cm-2 and a relative standard deviation of ±0.31 to ± 0.57%, depending on the concentration level. Beer's law is obeyed in the concentration range from 0.4 to 40 µg. ml-1 of thiamine hydrochloride. The present method has been developed for the determination of thiamine hydrochloride in the presence of sulphite. The proposed method has been applied successfully to the determination of vitamin B 1 in pharmaceutical preparations.
In this research, an easy, acurate and rapid spectrophotometric method for determination of sulphite and thiamine hydrochloride is described. This method used proton transfer reaction between sulphite ion with alizarine at (pH 6.73) to develop red colour adduct that bears maximum absorption at 524 nm. This method was developed to determination of thiamine hydrochloride by bleaching the red colour (alizarine-sulphite product) at the optimum conditions established for the determination of sulphite. Beer’s law is obeyed in the concentration range (10-1000) μg/25ml (0.4-40) of sulphite and 10-1500, μg/25ml (0.4-60) for thiamine hydrochloride at the selected wavelength, the molar absorbtivity 2.6×104 l.mol-1.cm-1 for SO32- and 3.37×104 l.mol-1.cm-1 for thiamine hydrochloride, Sandell's index for sensitivity 0.00307 μg .cm-2 for sulphite and 0.0100 μg .cm-2 for B1, the precision (RSD) of the method is ±0.23- ± 0.51% sulphite and ±0.15- ±0.25 thiamine hydrochloride. Good recoveries of sulphite and thiamine hydrochloride from water samples and pharmaceutical formation are achieved using the proposed method.
A simple, rapid and direct spectrophotometric method for the determination of beryllium II is described. The method is based on the reaction beryllium II with chrome azurol S in the presence of cetylpyridinium chloride at pH 5.0 forming a stable blue coloured complex which has an intense absorption at 620 nm. Beer's law was obeyed over the range of 0.25-100 µg/25 ml. The molar absorptivity and Sandal's sensitivity of the coloured complex are 1.02×10 4 l.mol-1 .cm-1 and 0.00883 µg.cm-2 , respectively. The interference caused by a number of common cations and anions can be removed by using EDTA as a masking agent. The method has been applied successfully to the determination of beryllium II in different water samples.
In this research, an easy, acurate and rapid spectrophotometric method for determination of sulphite and thiamine hydrochloride is described. This method used proton transfer reaction between sulphite ion with alizarine at (pH 6.73) to develop red colour adduct that bears maximum absorption at 524 nm. This method was developed to determination of thiamine hydrochloride by bleaching the red colour (alizarine-sulphite product) at the optimum conditions established for the determination of sulphite. Beer’s law is obeyed in the concentration range (10-1000) μg/25ml (0.4-40) of sulphite and 10-1500, μg/25ml (0.4-60) for thiamine hydrochloride at the selected wavelength, the molar absorbtivity 2.6×104 l.mol-1.cm-1 for SO32- and 3.37×104 l.mol-1.cm-1 for thiamine hydrochloride, Sandell's index for sensitivity 0.00307 μg .cm-2 for sulphite and 0.0100 μg .cm-2 for B1, the precision (RSD) of the method is ±0.23- ± 0.51% sulphite and ±0.15- ±0.25 thiamine hydrochloride. Good recoveries of sulphite and thiamine hydrochloride from water samples and pharmaceutical formation are achieved using the proposed method.
http://dx.doi.org/10.25130/tjps.24.2019.012
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