Effective screening of infectious
diseases requires a fast, cheap,
and population-scale testing. Antigen pool testing can increase the
test rate and shorten the screening time, thus being a valuable approach
for epidemic prevention and control. However, the overall percent
agreement (OPA) with polymerase chain reaction (PCR) is one-half to
three-quarters, hampering it from being a comprehensive method, especially
pool testing, beyond the gold-standard PCR. Here, a multiantibodies
transistor assay is developed for sensitive and highly precise antigen
pool testing. The multiantibodies capture SARS-CoV-2 spike S1 proteins
with different configurations, resulting in an antigen-binding affinity
down to 0.34 fM. The limit of detection reaches 3.5 × 10–17 g mL–1SARS-CoV-2 spike S1 protein
in artificial saliva, 4–5 orders of magnitude lower than existing
transistor sensors. The testing of 60 nasopharyngeal swabs exhibits
∼100% OPA with PCR within an average diagnoses time of 38.9
s. Owing to its highly precise feature, a portable integrated platform
is fabricated, which achieves 10-in-1 pooled screening for high testing
throughput. This work solves the long-standing problem of antigen
pool testing, enabling it to be a valuable tool in precise diagnoses
and population-wide screening of COVID-19 or other epidemics in the
future.
A metal-free procedure for the synthesis of thioethers is described via the base-promoted reductive coupling of tosylhydrazones with thiols through an insertion of a carbene into the S-H bond.
An FeCl 3 -catalyzed tandem reaction of 2-iodoaniline with isothiocyanate in water is described, which provides an environmentally benign, efficient, and practical route for the generation of 2-aminobenzothiazole. This present tandem process shows broad substrate scope in the presence of octadecyltrimethylammonium chloride as a phase-transfer catalyst. In addition, the reaction media can be recovered and recycled without loss of efficiency.
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