The breeding system of Banksia ilicifolia was assessed by performing controlled hand-pollination manipulations on flowers in a natural population in Perth, Western Australia. The percentage of 2000 flowers per treatment converted to fruits and seeds was assessed across 24 recipient plants following (1) self-pollination, (2) local outcross pollination (same population), (3) non-local outcross pollination (pollen sourced from another population 30 km away), (4) unpollinated but bagged flowers and (5) unpollinated, unbagged flowers (natural pollination). The relative performance of the resulting seeds was assessed by seed weight, germination rates and, in an unplanned component of the study, resistance to a fungal pathogen. The percentage of flowers converted to fruits following self-pollination was low (0.9%), but demonstrated self-compatibility. Fruit set following cross-pollinations (3.6 and 3.3% for non-local and local crosses, respectively) was significantly greater than that following self-pollination, open-pollination (0.4%) and autogamous (0.04%) treatments. Low fruit set for open-pollinated flowers, compared with self- and outcross-pollination treatments, suggests pollen limitation. Pollen tubes were observed in 15 and 20% of upper styles of flowers hand-pollinated with self and local outcross pollen, respectively. Seed germination was dependent on the source of pollen, where fewer selfed seeds germinated (37%) than did both non-local and local outcrossed seeds (83 and 91%, respectively). Selfed seedlings showed poorer survival (33.3%) following fungal attack than both non-local and local outcrossed seeds (69.2 and 68.5%, respectively). Only 13% of selfed seeds survived to be 2-month-old seedlings, compared with 63% for non-local and 57% for local outcrossed seeds. Ultimately, for 2000 flowers hand-pollinated with self pollen, only three seedlings survived to an age of 16 weeks, compared with 37 and 45 seedlings for local-cross and non-local cross treatments on 2000 hand-pollinated flowers, respectively. These results indicate that in this population, B. ilicifolia is self-compatible, but preferentially outcrossing, with strong early acting inbreeding depression. Consequently, the breeding system of B. ilicifolia promotes the maintenance of genetic variation and a high genetic load.
To assess whether wide outcrossing (over 30 km) in the naturally fragmented Banksia ilicifolia R.Br. increases the ecological amplitude of offspring, we performed a comparative greenhouse growth study involving seedlings of three hand‐pollinated progeny classes (self, local outcross, wide outcross) and a range of substrates and stress conditions. Outcrossed seedlings outperformed selfed seedlings, with the magnitude of inbreeding depression as high as 62% for seed germination and 37% for leaf area. Wide outcrossed seedlings outperformed local outcrossed seedlings, especially in non‐native soils, facilitated in part by an improved capacity to overcome soil constraints through greater root carboxylate exudation. Soil type significantly affected seedling growth, and waterlogging and water deficit decreased growth, production of cluster roots, root exudation and total plant P uptake. Our results suggest that the interaction of narrow ecological amplitude and the genetic consequences of small fragmented populations may in part explain the narrow range of local endemics, but that wide outcrossing may provide opportunities for increased genetic variation, increased ecological amplitude and range expansion.
Various reports of molecular genetic diversity evaluation of physic nut (J. curcas) have given inconsistent results. Part of the reasons were because of the used of unrealiable markers. This study was conducted to evaluate genetic diversity of Indonesian physic nut germplasm using four types of molecular markers (RAPD, ISSR, SSR and SCAR markers). Twenty four J. curcas accessions planted in Pakuwon, Sukabumi, with various phenotypes were evaluated. Twenty eight SSR marker loci yielded monomorphic allele pattern and indicated that the evaluated accessions probably were all genetically homogeneous for the respective loci. Eight RAPD and 4 ISSR primers out of the total 31 tested primers produced scoreable markers and some (i.e. UBC 873, OPG 17, OPP 03 and OPQ 11 primers) generated polymorphics markers. Genetic similarity coefficiens among evaluated accessions ranged from 0.6 to 1.0 with a population mean of 0.9 indicating low diversity and narrow genetic background among accessions in all populations. Therefore, breeding program utilizing such population would only result in low genetic gain. Based on the evaluated SCAR markers, all accessions belonged to the non-toxic Mexican type of physic nut. This information is important inputs for designing future physic nut breeding strategies in Indonesia.
Sugarcane development in Indonesia has been done primarily on dry lands. Therefore developing varieties more suitable to dry-agro-ecological conditions is being attempted as it is easily applicable and economically feasible. This study aimed at assessing the yield of 18 potential sugarcane clones, resulting from crosses with parents which have high productivity. This study used Cenning variety as a control. The research was conducted at Karangploso experimental garden Malang, from October 2019 to September 2020. The research used a randomized block design with four replications. The experimental plot was designed with five rows (5 m length) and rows distance at 110 cm. The evaluation was carried out on the growth and production component. The results showed that genotypes affected the performances of growth and yield characters. All of the clones showed good performances in growth. Clones MLG 18/21/14, MLG 18/42/15 and MLG 18/41/5 had productivity 21.08-34.86 (t/ha) and sucrose content 21.79-38.44 (%) higher than control. The three clones select as promising clones for sugarcane development in dry-agro-ecological land with higher productivity yields.
<p>ABSTRAK</p><p>Di Indonesia, jarak pagar (Jatropha curcas L.) merupakan tanamanpenting untuk menghasilkan biofuel. Dari biji tanaman ini dapatdiekstraksi bahan bakar biokerosen dan biodiesel. Tetapi potensi hasiltanaman ini perlu ditingkatkan agar pemanfaatannya dapat mempunyainilai komersial. Peningkatan potensi hasil tanaman jarak pagar dapatdilakukan dengan program pemuliaan tanaman. Tujuan penelitian yangdilakukan adalah mengevaluasi karakter vegetatif dan generatif sertapotensi hasil dari individu J. curcas hasil seleksi di Kebun Induk JarakPagar Pakuwon, Sukabumi. Penelitian ini juga mempelajari korelasi antarakarakter vegetatif dan generatif serta potensi hasil dari tanaman yangdievaluasi. Data yang diperoleh selanjutnya digunakan untuk meng-identifikasi individu tanaman yang dapat dimanfaatkan sebagai tetuapotensial untuk program pengembangan kultivar J. curcas yang berdayahasil tinggi. Tanaman yang dievaluasi berasal dari 20 genotipe terpilihyang berasal dari Lampung, Jawa Tengah, Jawa Timur, Nusa TenggaraBarat dan Sulawesi. Genotipe-genotipe tersebut diseleksi berdasarkan hasiluji provenan yang telah dilakukan sebelumnya. Dari setiap genotipeterpilih diambil tiga butir benih. Benih-benih tersebut kemudiandikecambahkan di tempat pembibitan yang terkontrol dan setelah berumur2 bulan bibit ditanam di lapangan pada tanggal 22 Mei 2007. Karaktervegetatif dan generatif serta potensi hasil dari individu-individu yangdievaluasi dimonitor selama satu tahun (1 Agustus 2007 – 31 Juli 2008).Hasil penelitian yang dilakukan menunjukkan tanaman-tanaman yang diuji(merupakan keturunan dari 20 genotipe J. curcas hasil seleksi), bervariasipada karakter tinggi tanaman, lingkar batang, dan percabangan. Tanaman-tanaman tersebut juga menunjukkan variasi pada umur berbunga, jumlahinfloresen, jumlah tandan buah, jumlah buah, dan jumlah biji serta kadarminyak biji. Tinggi tanaman, jumlah infloresen, dan jumlah tandan buahberkorelasi positif dengan hasil buah dan biji per tanaman. Sementara ituumur berbunga berkorelasi negatif dengan semua karakter generatif.Berdasarkan karakter vegetatif dan generatif serta potensi hasilnya,teridentifikasi tiga individu yang berpotensi sebagai tetua untukpengembangan kultivar J. curcas yang berdaya hasil tinggi. Individu-individu tersebut adalah HS 49-2, PT 13-2, dan PT 15-1. Ketiga individutersebut menghasilkan lebih dari 350 buah per tanaman dan kadarminyaknya berkisar antara 36 – 38%.</p><p>Kata kunci : Jatropha curcas L., evaluasi lapangan, seleksi fenotip,analisis korelasi, karakter vegetatif, karakter generatif, dayahasil, kandungan minyak biji</p><p>ABSTRACT</p><p>Morphologies and Yield Performances of 60 SelectedGenotypes of Physic nut (Jatropha curcas L.) at PakuwonExperimental Station, Sukabumi</p><p>Physic nut (Jatropha curcas L) is an important biofuelproducing crops in Indonesia. Biokerosene and biodiesel fuel could beextracted from seeds of this crop. Yield potential of this crop needs to beincreased in order to meet its commercial usage. Such yield potentialimprovement could be achieved through plant breeding program. Theobjectives of this research were to evaluate vegetative and generativecharacters and yield potentials of selected individual of J. curcas at KebunInduk Jarak Pagar Pakuwon, Sukabumi. This research was also intended toanalyze correlation among vegetative and generative characters and yieldpotentials of the evaluated genotypes. Subsequently, the collected datawere used to identify individual crop that can be used as potential parentgenotypes for developing new high yielding J. curcas cultivar. Theevaluated genotypes were derived from 20 selected parent plantsoriginated from Lampung, Central Java, East Java, West Nusa Tenggara,and Sulawesi. The genotypes were selected based on provenance trials inthe previous experiment. Three seeds were collected from each parentplant and were germinated in the controlled seed nursery conditions. Theseedlings were planted in the field on May 22, 2007 when they were 2months old. Vegetative and generative characters and yield potentials ofthe evaluated individuals were monitored for one year (August 1, 2007 toJuly 31, 2008). Results of the experiments indicated the tested genotypesderived from 20 selected J. curcas parent plants exhibited variabilities intheir height, stem girth, and branches characters. They also exhibited highvariabilities in their time of flowering, number of inflorescence, number ofbunches, number of fruits and nut per plant, and their oil contents. Plantheight, number of inflorescence, and number of bunches were positivelycorrelated with yield of fruits and nuts per plants. Meanwhile, time offlowering was negatively correlated with all generative characters. Basedon their vegetative and generative characters and their yield potentials,three genotypes were identified as the new potential parents for developinghigh yielding J. curcas cultivar. They were HS 49-2, PT 13-2, and PT 15-1. These newly selected genotypes yielded more than 350 fruits per plantwith 36 – 38 % seed oil content in their first year.</p><p>Key words : Jatropha curcas L., field evaluation, phenotypic selection,correlation analysis, vegetative characters, generativecharacters, seed yield, oil content</p>
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