To study the microbial population and fermentation dynamics of large needlegrass (LN) and Chinese leymus (CL) during ensiling and subsequent exposure to air, silages were sampled and analyzed using culture-based techniques and denaturing gradient gel electrophoresis (DGGE). A total of 112 lactic acid bacteria (LAB) strains were isolated and identified using the 16S rRNA sequencing method. Lactic acid was not detected in the first 20 days in LN silage and the pH decreased to 6.13 after 45 days of ensiling. The temperature of the LN silage increased after approximately 30 h of air exposure and the CL silage showed a slight temperature variation. Enterococcus spp. were mainly present in LN silage. The proportion of Lactobacillus brevis in CL silage increased after exposure to air. LN silage with a higher proportion of Enterococcus spp. and propionic acid concentration did not show higher fermentation quality or aerobic stability than CL silage, which had a higher concentration of acetic acid, butyric acid and increased proportion of L. brevis after exposure to air.
The findings demonstrate that culture-independent microbiota analysis may be useful in the isolation and identification of nonconventional LAB species involved in fermentation and the aerobic stability of silage.
Leymus chinensis is an important crop that can be fed to ruminants. The purpose of this study was to investigate the roles of Lactiplantibacillus plantarum and Lentilactobacillus buchneri in fermentation quality, aerobic stability, and dynamics of wilted L. chinensis silage microorganisms. Wilted L. chinensis silages were ensiled with/without L. plantarum and L. buchneri. After 14 and 56 days of ensiling, the silos were opened and subjected to a 7-day aerobic deterioration test. This study looked at the composition of fermentation products as well as the microbial communities in silage. Silage inoculated with L. plantarum and L. buchneri had an increased lactic acid content as well as lactic acid bacterial (LAB) quantity, but a decrease in pH and levels of butyric acid, 2,3-butanediol, and ethanol was observed during ensiling. Non-treated and L. plantarum-treated silages deteriorated in the 7-day spoilage test after opening day-14 silos, whereas L. buchneri-inoculated silage showed no signs of deterioration. Lactobacillus abundance increased in the 7-day spoilage test after opening day-56 silos, while undesirable microorganisms such as Acetobacter, Bacillus, and molds, namely, Aspergillus and Penicillium were inhibited within L. plantarum- and L. buchneri-inoculated silages. The composition of fermentation products was related to the bacterial community, particularly Lactobacillus, Enterococcus, and Acetobacter. To summarize, L. plantarum- and L. buchneri-inoculated silage enhanced fermentation quality during ensiling and inhibited aerobic spoilage in a 7-day spoilage test of 56 days ensiling within wilted L. chinensis silage.
The apparent metabolizable energy (
AME
), AME corrected to zero-nitrogen retention (
AMEn
), and net energy (
NE
) values of 2 corn samples both stored for 3 yr were determined in laying hens with reference diet substitution method. Reference diet was formulated according to standard layer requirement, and test diets contained 50% of corn samples and 50% of the reference diet. Fifty-four Hy-Line Brown hens at the age of 36 wk were used. The heat production and energy metabolism of birds were measured in open-circuit respiratory chambers with 6 replicates (3 birds per replicate) per diet in a randomized design. Birds were fed experimental diets for 7 D in the chamber as adaptation. During the following 3 D, feed intake, metabolizable energy value, nitrogen balance, energy balance, egg production, O
2
consumption, CO
2
production, and energy efficiency were determined. The AME values of corn 1 and corn 2 were 3,485 and 3,675 kcal/kg DM, respectively. The corresponding AMEn values were 3,452 and 3,596 kcal/kg DM, and the NE values were 2,575 and 2,693 kcal/kg DM, respectively. The NE:AME ratios of corn 1 and corn 2 were 74.4 and 73.3%, respectively. The NE:AMEn ratios of corn 1 and corn 2 were 75.0 and 74.9%, respectively. The AME, AMEn, and NE values of the 2 corn samples both stored for 3 yr were lower than the literature values for fresh corn.
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