The effect of 5 levels of nitrogen (0, 50, 100, 150 and 200 kg/ha) on herb yield, content and composition of essential oil of Tagetes minuta was studied in Tehran, Iran. Application of nitrogen fertilizer significantly increased the herb yield, content and percentages of constituents of essential oil of T. minuta. The largest fresh and dry herb yields (70 and 29 t/ha, respectively) and the highest essential oil content (1.16%) were obtained from the plots that received 200 kg N/ha. GC and GC-MS analyses of the essential oil showed that different levels of nitrogen had significant effect on the essential oil constituents of T. minuta. The major components of the essential oil were:
Collection stage of the flowers of feverfew had a significant effect on the essential oil content and compositions. The highest mean of essential oil content (0.55%) was extracted from the fruits which collected at fruit set stage, while the lowest mean essential oil of flowers (0.35%) was obtained from the flowers which collected at budding step. The results of GC and GC/MS analysis showed that 21, 19 and 21 compositions identified from budding, full flowering and fruit set stages respectively. The essential oil compositions of various harvest times of feverfew flowers are different, the fluctuations of amount of the main components the oil such as camphor, chrynsanthenyl acetae, camphene and α-pinene is not too high.
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