Recently, several mass spectrometry-and protein denaturation-based proteomic methods have been developed to facilitate protein-target discovery efforts in drug mode-of-action studies. These methods, which include the Stability of Proteins from Rates of Oxidation (SPROX), Pulse Proteolysis (PP), Chemical Denaturation and Protein Precipitation (CPP), and Thermal Proteome Profiling (TPP) techniques, have been used in an increasing number of applications in recent years. However, while the advantages and disadvantages to using these different techniques have been reviewed, the analytical characteristics of these methods have not been directly compared.Reported here is such a direct comparison using the well-studied immuno-suppressive drug, cyclosporine A (CsA), and the proteins in a yeast cell lysate. Also described is a one-pot strategy that can be utilized with each technique to streamline data acquisition and analysis. We find that there are benefits to utilizing all four strategies for protein target discovery including increased proteomic coverage and reduced false positive rates that approach 0%. Moreover, the one-pot strategy described here makes such an experiment feasible, because of the 10-fold reduction in reagent costs and instrument time it affords.
To the Editor, The human exposome contains millions of proteins, yet only a select few consistently become allergens. This has led to speculation that there are intrinsic factors which differentiate allergens from non-allergens. Anecdotal evidence suggested that abundance and stability could be two such properties, but no rigorous statistical comparisons were available until a study of the dust mite Dermatophagoides pteronyssinus (Dp) combined transcriptomics with proteomics. 1 The Dp study measured transcription levels as a proxy for protein abundance and correlated the results with proteome-wide stability measurements using mass spectrometry. It was found that the mite allergens were as a population more stable and more highly expressed than Dp non-allergens. Dp was a good model
A scale of relative affinities of a series of 2'-deoxycytidine and cytidine (CD) derivatives was established based on the data of cross-reactivities of these compounds as well as the displacements obtained from a competitive ELISA. No correlation could be established between the nucleosides modifying structures and the affinities. This can be explained by the possibilities of the modifying structures of intra- and intermolecular nonimmunospecific interactions owing to their degree of functionalization.
Pollen calendars are practical instruments for allergists. The purpose of this study was to quantify and describe different levels of airborne pollen in our working-area (Infanta Leonor U.H, Madrid). METHODS: We monitored the pollen in our area (January 2017-December 2019) with a Hirst-type volumetric collector, according to the guidelines of the Aerobiology Committee of the Spanish Academy of Asthma and Clinical Immunology. RESULTS: Pollen counts divided by families were as follows:
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